Summary
N-glycosylation is a maturation event necessary for the correct function, efficiency, and stability of a high number of biopharmaceuticals. This chapter presented here proposes various methods to determine whether, how, and where a plant pharmaceutical is N-glycosylated. These methods rely on blot detection with glycan-specific probes, specific deglycosylation of glycoproteins followed by mass spectrometry, N-glycan profile analysis, and glycopeptide identification by LC-MS.
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Acknowledgments
We thank present and former colleagues from our lab who contributed to the development and optimization of the strategies described in this manuscript. Work on glycobiology at the University of Rouen is supported by the French Ministère de la Recherche and the Centre National de la Recherche Scientifique (CNRS). J.A.C. and G.C. gratefully thank the support and collaboration between Biomedical Research and AgroBiotech Directions from the Center for Genetic Engineering and Biotechnology, Havana, Cuba.
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Bardor, M. et al. (2009). N-Glycosylation of Plant Recombinant Pharmaceuticals. In: Faye, L., Gomord, V. (eds) Recombinant Proteins From Plants. Methods in Molecular Biology™, vol 483. Humana Press. https://doi.org/10.1007/978-1-59745-407-0_14
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DOI: https://doi.org/10.1007/978-1-59745-407-0_14
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