Abstract
B-cells and antibody-secreting plasma cells are key players in protective immunity, but also in autoimmune disease. To understand their various functions in the initiation and maintenance of autoimmune pathology, a detailed dissection of their functional diversity is mandatory. This requires a detailed phenotypic classification of the diversity of B-cells. Here, technologies of immunocytometry and ELISpot are described in detail, and their value for phenotypic characterization of cells of the B lineage, as well as for preparative cell sorting, to further characterize them functionally and on the molecular level are described.
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Mei, H.E. et al. (2007). Phenotypic Analysis of B-Cells and Plasma Cells. In: Cope, A.P. (eds) Arthritis Research. Methods in Molecular Medicine, vol 136. Humana Press. https://doi.org/10.1007/978-1-59745-402-5_1
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DOI: https://doi.org/10.1007/978-1-59745-402-5_1
Publisher Name: Humana Press
Print ISBN: 978-1-58829-918-5
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