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Hepatitis C pp 55–71Cite as

A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 510))

Abstract

The HCV genome is highly heterogeneous; more and more genotypes, each with several distinct subtypes, are being identified around the world. Knowledge of genotype is important for planning of treatment regimes, whereas subtype identification is useful in epidemiological studies and outbreak investigation. We describe HCV genotyping and subtyping assays, based on real-time PCR, that are sensitive, specific, and reliable. These assays provide fast, accurate, and convenient methods for HCV genotyping/subtyping to support clinical practice.

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Author information

Authors and Affiliations

  1. Health Protection Agency Cambridge Microbiology and Public Health Laboratory Addenbrooke’s Hospital, Cambridge, UK

    Kathryn J. Rolfe, Tim G. Wreghitt, Graeme J. M. Alexander & Martin D. Curran

Authors
  1. Kathryn J. Rolfe
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  2. Tim G. Wreghitt
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  3. Graeme J. M. Alexander
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  4. Martin D. Curran
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Editor information

Editors and Affiliations

  1. Florida State University, Tallahassee, FL, USA

    Hengli Tang

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Rolfe, K.J., Wreghitt, T.G., Alexander, G.J.M., Curran, M.D. (2009). A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates. In: Tang, H. (eds) Hepatitis C. Methods in Molecular Biology™, vol 510. Humana Press. https://doi.org/10.1007/978-1-59745-394-3_5

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  • DOI: https://doi.org/10.1007/978-1-59745-394-3_5

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-970-3

  • Online ISBN: 978-1-59745-394-3

  • eBook Packages: Springer Protocols

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