Skip to main content
SpringerLink
Log in
Book cover

Macromolecular Crystallography Protocols, Volume 1

Preparation and Crystallization of Macromolecules

  • Book
  • © 2007

Overview

Editors:
  1. John M. Walker
    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Sylvie Doublié

    1. Department of Microbiology and Molecular Genetics, University of Vermont, Burlington

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 363)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (13 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag

    • Joseph E. Tropea, Scott Cherry, Sreedevi Nallamsetty, Christophe Bignon, David S. Waugh
    Pages 1-19

  3. Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project

    • Sophie Quevillon-Cheruel, Bruno Collinet, Lionel Trésaugues, Philippe Minard, Gilles Henckes, Robert Aufrère et al.
    Pages 21-37

  4. Baculoviral Expression of an Integral Membrane Protein for Structural Studies

    • Dean R. Madden, Markus Safferling
    Pages 39-57

  5. Protein Engineering

    • Sonia Longhi, François Ferron, Marie-Pierre Egloff
    Pages 59-90

  6. Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems

    • Sylvie Doublié
    Pages 91-108

  7. How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals

    • Gloria E. O. Borgstahl
    Pages 109-130

  8. Screening and Optimization Methods for Nonautomated Crystallization Laboratories

    • Terese Bergfors
    Pages 131-152

  9. Improving Marginal Crystals

    • Charles W. Carter Jr., Madeleine Riès-Kautt
    Pages 153-174

  10. Optimization Techniques for Automation and High Throughput

    • Naomi E. Chayen
    Pages 175-190

  11. Three-Dimensional Crystallization of Membrane Proteins

    • James Féthière
    Pages 191-223

  12. Crystallization of Protein-DNA Complexes

    • Thomas Hollis
    Pages 225-237

  13. Preparation and Crystallization of RNA

    • Barbara L. Golden
    Pages 239-257

  14. Crystallization of RNA-Protein Complexes

    • Eiji Obayashi, Chris Oubridge, Daniel Pomeranz Krummel, Kiyoshi Nagai
    Pages 259-276

  15. Back Matter

    Pages 277-279
    Download chapter PDF
Back to top

Keywords

About this book

Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators.

Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.

Editors and Affiliations

  • Department of Microbiology and Molecular Genetics, University of Vermont, Burlington

    Sylvie Doublié

Bibliographic Information

  • Book Title: Macromolecular Crystallography Protocols, Volume 1

  • Book Subtitle: Preparation and Crystallization of Macromolecules

  • Editors: John M. Walker, Sylvie Doublié

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-209-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-292-6Published: 05 February 2007

  • Softcover ISBN: 978-1-61737-475-3Published: 10 November 2010

  • eBook ISBN: 978-1-59745-209-0Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 280

  • Number of Illustrations: 49 b/w illustrations

  • Topics: Biochemistry, general, Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation