Overview
- Provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations
- Offers a variety of transplantation approaches to measure HSC function in vivo
- Details retroviral and lentiviral transduction procedures and analysis of integration sites
- Describes assays using embryonic and adult tissues to derive HSC
Part of the book series: Methods in Molecular Biology (MIMB, volume 430)
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Keywords
Table of contents (20 protocols)
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Overview
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Stem Cell Enrichment and Analysis
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In Vitro Assays and Differentiation
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Transplantation Assays for Mouse and Human HSC
Editors and Affiliations
Bibliographic Information
Book Title: Hematopoietic Stem Cell Protocols
Editors: Kevin D. Bunting
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-182-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-868-3Published: 31 January 2008
Softcover ISBN: 978-1-61737-816-4Published: 16 December 2010
eBook ISBN: 978-1-59745-182-6Published: 04 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 312
Topics: Biochemistry, general, Cell Biology, Hematology, Molecular Medicine