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Preparation of Recombinant HIV-1 Gag Protein and Assembly of Virus-Like Particles In Vitro

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Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 485))

Abstract

The mechanism of assembly of retroviruses is not fully understood. Purification of retroviral Gag protein and studying its solution state and assembly properties might provide insights into retroviral assembly mechanisms. Here we describe a rapid method for the purification of Gag and its subsequent assembly into virus-like particles in a defined system in vitro. The purification scheme does not use affinity tags, but purifies the native protein by virtue of its high affinity for phosphocellulose, a property presumably related to the affinity of Gag proteins for nucleic acids.

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References

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Acknowledgments

This work was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Datta, S.A., Rein, A. (2009). Preparation of Recombinant HIV-1 Gag Protein and Assembly of Virus-Like Particles In Vitro. In: Prasad, V.R., Kalpana, G.V. (eds) HIV Protocols. Methods In Molecular Biology™, vol 485. Humana Press. https://doi.org/10.1007/978-1-59745-170-3_14

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  • DOI: https://doi.org/10.1007/978-1-59745-170-3_14

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-859-1

  • Online ISBN: 978-1-59745-170-3

  • eBook Packages: Springer Protocols

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