Abstract
Bioluminescent yeast assays BLYES and BLYAS are whole-cell bioassays that utilize genetically modified Saccharomyces cerevisiae bioreporters to detect estrogenic and androgenic activities, respectively. The bioreporter strains chromosomally express human estrogen receptor alpha (BLYES) or androgen receptor (BLYAS) and contain a reporter plasmid expressing the complete bacterial luciferase gene cassette (luxCDABE) under the control of an estrogen- or androgen-responsive promoter. Exposure to endocrine-disrupting compounds activates the receptor which subsequently turns on the expression of the reporter genes, resulting in dose-dependent bioluminescence (i.e., light) emission. These yeast whole-cell bioassays provide rapid, cost-effective, and high-throughput detection of endocrine-disrupting activities in environmental samples. This protocol will provide a detailed description of the standard assay procedures as well as a framework for data analysis.
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Acknowledgments
Research funding was provided by the National Institute of Food and Agriculture, US Department of Agriculture, under award number 2015-33610-23598 and the US National Science Foundation, Chemical, Bioengineering, Environmental and Transport Systems (CBET) program, under award number 1530953.
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Xu, T., Young, A., Narula, J., Sayler, G., Ripp, S. (2020). High-Throughput Analysis of Endocrine-Disrupting Compounds Using BLYES and BLYAS Bioluminescent Yeast Bioassays. In: Ripp, S. (eds) Bioluminescent Imaging. Methods in Molecular Biology, vol 2081. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9940-8_3
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DOI: https://doi.org/10.1007/978-1-4939-9940-8_3
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