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Biomarkers for Immunotherapy of Cancer

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Magdalena Thurin

    1. NIH, National Cancer Institute, Bethesda, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Alessandra Cesano

    1. Nanostring Technologies, Seattle, WA, USA, ESSA Pharma, South San Francisco, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Francesco M. Marincola

    1. Refuge Biotechnologies, Menlo Park, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2055)

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About this book

This book provides the immune oncology (IO) community with a deeper understanding of the scope of the biomarker methods to potentially improve the outcome from immunotherapy. The editors secured the input from experts in the field dedicated to translating scientific research from bench to bedside was submitted. The book provides not only details about the technical, standardization and interpretation aspects of the methods but also introduces the reader to the background information and scientific justification for selected biomarkers and assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xviii
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  2. Scientific Questions and the Status of Inhibitory Receptors for Immunotherapy

    1. Front Matter

      Pages 1-1
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    2. Status of Immune Oncology: Challenges and Opportunities

      • Alessandra Cesano, Francesco M. Marincola, Magdalena Thurin
      Pages 3-21

    3. Immunological Targets for Immunotherapy: Inhibitory T Cell Receptors

      • Diwakar Davar, Hassane M. Zarour
      Pages 23-60

  3. Mutational and Germline Profiling

    1. Front Matter

      Pages 61-61
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    2. Measuring Tumor Mutational Burden Using Whole-Exome Sequencing

      • Tomas Vilimas
      Pages 63-91

    3. Germline Genetics in Immuno-oncology: From Genome-Wide to Targeted Biomarker Strategies

      • Tomas Kirchhoff, Robert Ferguson
      Pages 93-117

    4. Detection of Microsatellite Instability Biomarkers via Next-Generation Sequencing

      • Russell Bonneville, Melanie A. Krook, Hui-Zi Chen, Amy Smith, Eric Samorodnitsky, Michele R. Wing et al.
      Pages 119-132

    5. Genetic Instability Markers in Cancer

      • Giuseppe Palmieri, Milena Casula, Antonella Manca, Grazia Palomba, Maria Cristina Sini, Valentina Doneddu et al.
      Pages 133-154

    6. Validation of Circulating Tumor DNA Assays for Detection of Metastatic Melanoma

      • Mahrukh M. Syeda, Jennifer M. Wiggins, Broderick Corless, Cindy Spittle, George Karlin-Neumann, David Polsky
      Pages 155-180

    7. Techniques Associated with Exosome Isolation for Biomarker Development: Liquid Biopsies for Ovarian Cancer Detection

      • Shayna Sharma, Carlos Salomon
      Pages 181-199

  4. Epigenetic Profiling

    1. Front Matter

      Pages 201-201
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    2. Identifying MicroRNA Pathway Variants as Biomarkers of Patient Selection for Immune Therapy

      • Joanne B. Weidhaas
      Pages 203-212

    3. Histone Modifications as Biomarkers for Immunotherapy

      • Erin M. Taylor, Brian Koss, Lauren E. Davis, Alan J. Tackett
      Pages 213-228

  5. Functional Genomic Profiling

    1. Front Matter

      Pages 229-229
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    2. Functional Genome Profiling to Understand Cancer Immune Responsiveness

      • Ena Wang, Davide Bedognetti, Francesco M. Marincola
      Pages 231-244

    3. Using RNA Sequencing to Characterize the Tumor Microenvironment

      • C. C. Smith, L. M. Bixby, K. L. Miller, S. R. Selitsky, D. S. Bortone, K. A. Hoadley et al.
      Pages 245-272

    4. Development of Gene Expression-Based Biomarkers on the nCounter® Platform for Immuno-Oncology Applications

      • Sarah Warren, Patrick Danaher, Afshin Mashadi-Hossein, Lynell Skewis, Brett Wallden, Sean Ferree et al.
      Pages 273-300

    5. The CRISPR System and Cancer Immunotherapy Biomarkers

      • Vitaly Balan, Jianbin Wang
      Pages 301-322

  6. Phenotyping of Tumor and Immune Cells

    1. Front Matter

      Pages 323-323
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Editors and Affiliations

  • NIH, National Cancer Institute, Bethesda, USA

    Magdalena Thurin

  • Nanostring Technologies, Seattle, WA, USA, ESSA Pharma, South San Francisco, USA

    Alessandra Cesano

  • Refuge Biotechnologies, Menlo Park, USA

    Francesco M. Marincola

Bibliographic Information

  • Book Title: Biomarkers for Immunotherapy of Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Magdalena Thurin, Alessandra Cesano, Francesco M. Marincola

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9773-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9772-5Published: 10 September 2019

  • Softcover ISBN: 978-1-4939-9775-6Published: 10 September 2020

  • eBook ISBN: 978-1-4939-9773-2Published: 09 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 733

  • Number of Illustrations: 8 b/w illustrations, 111 illustrations in colour

  • Topics: Cancer Research, Immunology

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