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Metabolic Profiling Using In Vivo High Field Flow NMR

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NMR-Based Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2037))

Abstract

In vivo NMR (nuclear magnetic resonance) has the potential to monitor and record metabolic flux in close to real time, which is essential for better understanding the toxic mode of action of a contaminant and deciphering complex interconnected stress-induced pathways impacted inside an organism. Here, we describe how to construct and use a simple flow system to keep small aquatic organisms alive inside the NMR spectrometer. In living organisms, magnetic susceptibility distortions lead to severe broadening in conventional NMR. Two main approaches can be employed to overcome this issue: (1) use a pulse sequence to reduce the distortions, or (2) employ multidimensional NMR in combination with isotopic enrichment to provide the spectral dispersion required to separate peaks from overlapping resonances. Both approaches are discussed, and protocols for both approaches are provided here in the context of small aquatic organisms.

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Acknowledgments

Andre Simpson would like to thank the Natural Sciences and Engineering Research Council (NSERC) (Strategic (STPGP 494273-16) and Discovery Programs (RGPIN-2019-04165)), the Canada Foundation for Innovation (CFI), the Ontario Ministry of Research and Innovation (MRI), the Krembil Foundation for providing funding, and the Government of Ontario for an Early Researcher Award.

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Authors and Affiliations

  1. Department of Physical and Environmental Sciences, University of Toronto Scarborough, Toronto, ON, Canada

    Maryam Tabatabaei Anaraki, Monica Bastawrous & André J. Simpson

  2. Department of Chemistry, University of Toronto Scarborough, Toronto, ON, Canada

    Daniel Lane, Amy Jenne & André J. Simpson

Authors
  1. Maryam Tabatabaei Anaraki
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  2. Daniel Lane
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  3. Monica Bastawrous
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  4. Amy Jenne
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  5. André J. Simpson
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Corresponding author

Correspondence to André J. Simpson .

Editor information

Editors and Affiliations

  1. Northwest Metabolomics Research Center, Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA

    G. A. Nagana Gowda

  2. Northwest Metabolomics Research Center, Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Fred Hutch Cancer Research Center, Seattle, WA, USA

    Daniel Raftery

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Anaraki, M.T., Lane, D., Bastawrous, M., Jenne, A., Simpson, A.J. (2019). Metabolic Profiling Using In Vivo High Field Flow NMR. In: Gowda, G., Raftery, D. (eds) NMR-Based Metabolomics. Methods in Molecular Biology, vol 2037. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9690-2_22

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  • DOI: https://doi.org/10.1007/978-1-4939-9690-2_22

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9689-6

  • Online ISBN: 978-1-4939-9690-2

  • eBook Packages: Springer Protocols

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