Abstract
1.8 μm Silica hydride particles have been derivatized with vancomycin and applied to the enantioseparation of some racemic herbicides and nonsteroidal anti-inflammatory drugs (NSAIDs) by nano-liquid chromatography. The chiral stationary phase (CSP) was packed for only 11 cm and the enantiomers were separated utilizing a laboratory-assembled instrumentation. The new CSP was very effective for the separation of the above mentioned acidic compounds, while poor resolutions were obtained for basic compounds. Mixtures of acetate buffer with methanol or acetonitrile allowed the chiral resolution of all compounds. Fast chiral separation of a NSAIDs-related compound can be achieved in less than 60 s.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
De-Miao C, Qiang F, Na L, Song-Xian Z, Qian-Qian Z (2007) Enantiomeric separation of naproxen by high performance liquid chromatography using CHIRALCEL OD as stationary phase. Chin J Anal Chem 35:75–78
D’Orazio G, Fanali C, Asensio-Ramos M, Fanali S (2017) Chiral separations in food analysis. TrAC—Trends Anal Chem 96:151–171
Armstrong DW, Tang YB, Chen SS et al (1994) Macrocyclic antibiotics as a new class of chiral selectors for liquid chromatography. Anal Chem 66:1473–1484
Armstrong DW, Rundlett K, Reid GL III (1994) Use of a macrocyclic antibiotic, rifamycin b, and indirect detection for the resolution of racemic amino alcohols by CE. Anal Chem 66:1690–1695
Ward TJ, Oswald TM (1997) Enantioselectivity in capillary electrophoresis using the macrocyclic antibiotics. J Chromatogr A 792:309–325
Desiderio C, Fanali S (1998) Chiral analysis by capillary electrophoresis using antibiotics as chiral selector. J Chromatogr A 807:37–56
Aboul-Enein HY, Ali I (2001) Macrocyclic antibiotics as effective chiral selectors for enantiomeric resolution by liquid chromatography and capillary electrophoresis. Chromatographia 52:679–691
Tang A-N, Wang X-N, Ding G-S, Yan X-P (2009) On-line preconcentration and enantioseparation of thalidomide racemates by CEC with the hyphenation of octyl and norvancomycin monoliths. Electrophoresis 30:682–688
D’Orazio G, Fanali S (2010) Coupling capillary electrochromatography with mass spectrometry by using a liquid-junction nano-spray interface. J Chromatogr A 1217:4079–4086
Rocchi S, Fanali C, Fanali S (2015) Use of a novel sub-2 μm silica hydride vancomycin stationary phase in nano-liquid chromatography. II. Separation of derivatized amino acid enantiomers. Chirality 27:767–772
Rocchi S, Rocco A, Pesek JJ, Matyska MT, Capitani D, Fanali S (2015) Enantiomers separation by nano-liquid chromatography: use of a novel sub-2μm vancomycin silica hydride stationary phase. J Chromatogr A 1381:149–159
D’Orazio G, Cifuentes A, Fanali S (2008) Chiral nano-liquid chromatography-mass spectrometry applied to amino acids analysis for orange juice profiling. Food Chem 108:1114–1121
D’Orazio G, Aturki Z, Cristalli M, Quaglia MG, Fanali S (2005) Use of vancomycin chiral stationary phase for the enantiomeric resolution of basic and acidic compounds by nano-liquid chromatography. J Chromatogr A 1081:105–113
Armstrong DW, Rundlett KL, Chen J-R (1994) Evaluation of the macrocyclic antibiotic vancomycin as a chiral selector for capillary electrophoresis. Chirality 6:496–509
Loukili B, Dufresne C, Jourdan E et al (2003) Study of tryptophan enantiomer binding to a teicoplanin-based stationary phase using the perturbation technique: investigation of the role of sodium perchlorate in solute retention and enantioselectivity. J Chromatogr A 986:45–53
Fanali S, Desiderio C (1996) Use of vancomycin as chiral selector in capillary electrophoresis. Optimization and quantitation. J High Resolut Chromatogr 19:322–326
Ward TJ, Dann C III, Brown AP (1996) Separation of enantiomers using vancomycin in a countercurrent process by suppression of electroosmosis. Chirality 8:77–83
Desiderio C, Aturki Z, Fanali S (2001) Use of vancomycin silica stationary phase in packed capillary electrochromatography I. Enantiomer separation of basic compounds. Electrophoresis 22:535–543
Fanali S, Rudaz S, Veuthey JL, Desiderio C (2001) Use of vancomycin silica stationary phase in packed capillary electrochromatography. II: Enantiomer separation of venlafaxine and O-desmethylvenlafaxine in human plasma. J Chromatogr A 919:195–203
Kotoni D, Ciogli A, Molinaro C et al (2012) Introducing enantioselective ultrahigh-pressure liquid chromatography (eUHPLC): theoretical inspections and ultrafast separations on a new sub-2-mm Whelk-O1 stationary phase. Anal Chem 84:6805–6813
Farooq Wahab M, Wimalasinghe RM, Wang Y et al (2016) Salient sub-second separations. Anal Chem 88:8821–8826
D’Orazio G, Rocco A, Fanali S (2012) Fast-liquid chromatography using columns of different internal diameters packed with sub-2 μm silica particles. J Chromatogr A1228:213–220
Chankvetadze B, Bergenthal D, Wennemer H. Vorrichtung zur Trennung von Substanzgemischen mittels Flüssigchromatographie. DE10260700. 2004.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Fanali, C., Fanali, S. (2019). Application of Sub-2 Micron Particle Silica Hydride Derivatized with Vancomycin for Chiral Separations by Nano-Liquid Chromatography. In: Scriba, G.K.E. (eds) Chiral Separations. Methods in Molecular Biology, vol 1985. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9438-0_13
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9438-0_13
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9437-3
Online ISBN: 978-1-4939-9438-0
eBook Packages: Springer Protocols