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Biofuels from Algae

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Kristian Spilling

    1. Finnish Environment Institute, Helsinki, Finland

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1980)

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About this book

This volume provides a comprehensive overview of different ways to grow algae and the techniques used to start algal cultivation, monitor algal growth, environmental impact of growing algae, and various methods for characterizing the biomass. The chapters in this book discuss a range of topics such as step-by-step protocols on how to isolate and grow algae; descriptions on how to use fluorescence to estimate algal biomass and lipid content; and instructions on using advanced techniques to determine the carbohydrates and lipids in algal biomass. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Biofuels from Algae: Methods and Protocols is a valuable resource for novice and experienced researchers who want to learn more about this impactful and developing field.



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Table of contents (23 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Large Scale Cultivation of Microalgae: Open and Closed Systems

    • Jacqueline Jerney, Kristian Spilling
    Pages 1-8

  3. Design and Analysis of Offshore Macroalgae Biorefineries

    • Alexander Golberg, Alexander Liberzon, Edward Vitkin, Zohar Yakhini
    Pages 9-33

  4. Basic Methods for Isolating and Culturing Microalgae

    • Kristian Spilling
    Pages 35-39

  5. Measurement of Fluorescence for Monitoring Algal Growth and Health

    • Kristian Spilling, Jukka Seppälä
    Pages 41-45

  6. Microalgae Lipid Staining with Fluorescent BODIPY Dye

    • Judita Koreivienė
    Pages 47-53

  7. Monitoring Microalgal Neutral Lipid Accumulation with Nile Red

    • Katariina Natunen
    Pages 55-61

  8. Determining Inorganic and Organic Carbon

    • Jaana Koistinen, Mervi Sjöblom, Kristian Spilling
    Pages 63-70

  9. Determining Inorganic and Organic Nitrogen

    • Jaana Koistinen, Mervi Sjöblom, Kristian Spilling
    Pages 71-80

  10. Total Nitrogen Determination by a Spectrophotometric Method

    • Jaana Koistinen, Mervi Sjöblom, Kristian Spilling
    Pages 81-86

  11. Determining Inorganic and Organic Phosphorus

    • Jaana Koistinen, Mervi Sjöblom, Kristian Spilling
    Pages 87-94

  12. Determining Dissolved and Biogenic Silica

    • Jaana Koistinen, Mervi Sjöblom, Kristian Spilling
    Pages 95-101

  13. Life Cycle Assessment of Seaweed Cultivation Systems

    • Michele Seghetta, Pietro Goglio
    Pages 103-119

  14. Life-Cycle Assessment (LCA) Analysis of Algal Fuels

    • Homa Hosseinzadeh-Bandbafha, Meisam Tabatabaei, Mortaza Aghbashlo, Alawi Sulaiman, Abbas Ghassemi
    Pages 121-151

  15. Metabolic Engineering of Microalgae for Biofuel Production

    • Mohammad Pooya Naghshbandi, Meisam Tabatabaei, Mortaza Aghbashlo, Muhammad Nauman Aftab, Irfana Iqbal
    Pages 153-172

  16. Analytical Grade Purification of Phycocyanin from Cyanobacteria

    • Mahammed Ilyas Khazi, Zeliha Demirel, Fakhra Liaqat, Meltem Conk Dalay
    Pages 173-179

  17. Complete Acid-Based Hydrolysis Assay for Carbohydrate Quantification in Seaweed: A Species-Specific Optimized Approach

    • Emily T. Kostas, Stuart J. Wilkinson, Daniel A. White, David J. Cook
    Pages 181-190

  18. Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography

    • S. Van Wychen, L. M. L. Laurens
    Pages 191-202

  19. Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In Situ Transesterification

    • S. Van Wychen, L. M. L. Laurens
    Pages 203-214

  20. Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Samples

    • Heli Nygren, Tuulikki Seppänen-Laakso, Heiko Rischer
    Pages 215-222
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Editors and Affiliations

  • Finnish Environment Institute, Helsinki, Finland

    Kristian Spilling

Bibliographic Information

  • Book Title: Biofuels from Algae

  • Book Subtitle: Methods and Protocols

  • Editors: Kristian Spilling

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9416-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9415-1Published: 13 December 2019

  • Softcover ISBN: 978-1-4939-9418-2Published: 11 December 2020

  • eBook ISBN: 978-1-4939-9416-8Published: 11 December 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 249

  • Number of Illustrations: 13 b/w illustrations, 33 illustrations in colour

  • Topics: Biotechnology, Plant Sciences

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