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Microcalorimetry of Biological Molecules pp 169–183Cite as

Analysis of Isothermal Titration Calorimetry Data for Complex Interactions Using I2CITC

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1964))

Abstract

I2CITC allows the analysis of isothermal titration calorimetry (ITC) data for complex coupled equilibria. Here we describe how, using I2CITC, ITC data for systems involving a self-aggregating ligand and a host offering one or two binding sites can be analyzed, how interaction models can be tested, and how confidence intervals for the optimized parameters can be determined.

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Acknowledgment

Users of ICITC and I2CITC, past and present, are thanked for their feedback and questions which have contributed to the improvement of the software over the years.

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Authors and Affiliations

  1. Department of Chemistry, College of Science, Salahaddin University, Erbil, Kurdistan Region, Iraq

    Ibrahim Q. Saeed

  2. Physical Organic Chemistry Centre, School of Chemistry, Cardiff University, Cardiff, UK

    Niklaas J. Buurma

Authors
  1. Ibrahim Q. Saeed
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  2. Niklaas J. Buurma
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Corresponding author

Correspondence to Niklaas J. Buurma .

Editor information

Editors and Affiliations

  1. Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, CNRS, Strasbourg, France

    Eric Ennifar

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Saeed, I.Q., Buurma, N.J. (2019). Analysis of Isothermal Titration Calorimetry Data for Complex Interactions Using I2CITC. In: Ennifar, E. (eds) Microcalorimetry of Biological Molecules. Methods in Molecular Biology, vol 1964. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9179-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9179-2_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9178-5

  • Online ISBN: 978-1-4939-9179-2

  • eBook Packages: Springer Protocols

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