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Monitoring α-Synuclein Proteotoxicity in Drosophila Models

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Book cover Alpha-Synuclein

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1948))

Abstract

Parkinson’s disease is the second most common neurodegenerative disease without cure. It is characterized by α-synuclein accumulation and aggregation in dopaminergic and other types of neurons. Because α-synuclein accumulation leads to a toxic gain of function, its ectopic expression in Drosophila has been a useful in vivo model for testing modifiers of its toxicity. This chapter describes four assays: the rapid iterative negative geotaxis, rough eye phenotype, quantification of dopaminergic neuronal loss, and measurements of circadian effects.

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Acknowledgements

GKT is funded by a Wellcome Trust Intermediate Clinical Fellowship (097479/Z/11/Z), the Wellcome Beit Prize (097479/Z/11/A), and the NIHR Oxford Biomedical Research Centre.

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Authors and Affiliations

  1. Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary

    Aron Szabo

  2. Nuffield Department of Clinical Neurosciences, University of Oxford, John Radcliffe Hospital, Oxford, UK

    George K. Tofaris

Authors
  1. Aron Szabo
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  2. George K. Tofaris
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Corresponding author

Correspondence to George K. Tofaris .

Editor information

Editors and Affiliations

  1. Dementia Research Institute, University College London, London, UK

    Tim Bartels

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Szabo, A., Tofaris, G.K. (2019). Monitoring α-Synuclein Proteotoxicity in Drosophila Models. In: Bartels, T. (eds) Alpha-Synuclein. Methods in Molecular Biology, vol 1948. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9124-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9124-2_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9123-5

  • Online ISBN: 978-1-4939-9124-2

  • eBook Packages: Springer Protocols

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