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Isolation and Culture of Primary Mouse Middle Ear Epithelial Cells

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Book cover Mouse Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1940))

Abstract

Epithelial abnormalities underpin the development of the middle ear disease, otitis media (OM). Until now, a well-characterized in vitro model of the middle ear (ME) epithelium that replicates the complex cellular composition of the middle ear has not been available. This chapter describes the development of a novel in vitro model of mouse middle ear epithelial cells (mMECs), cultured at the air-liquid interface (ALI). This system enables recapitulation of the characteristics of the native murine ME epithelium. We demonstrate that mMECs undergo differentiation into the varied cell populations seen within the native middle ear. Overall, our mMEC culture system can help better understand the cell biology of the middle ear and improve our understanding of the pathophysiology of OM. The model also has the potential to serve as a platform for validation of treatments designed to reverse aspects of epithelial remodeling underpinning OM development.

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Acknowledgments

This work was supported by a University of Sheffield PhD Studentship (supervised by LB and CDB) and funds from MRC Harwell.

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Authors and Affiliations

  1. Academic Unit of Respiratory Medicine, Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, UK

    Apoorva Mulay, Khondoker Akram & Colin D. Bingle

  2. Oral and Maxillofacial Pathology, Department of Clinical Dentistry, University of Sheffield, Sheffield, UK

    Lynne Bingle

Authors
  1. Apoorva Mulay
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  2. Khondoker Akram
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  3. Lynne Bingle
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  4. Colin D. Bingle
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Corresponding author

Correspondence to Colin D. Bingle .

Editor information

Editors and Affiliations

  1. Lung Health Research Centre, Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, VIC, Australia

    Ivan Bertoncello

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Mulay, A., Akram, K., Bingle, L., Bingle, C.D. (2019). Isolation and Culture of Primary Mouse Middle Ear Epithelial Cells. In: Bertoncello, I. (eds) Mouse Cell Culture. Methods in Molecular Biology, vol 1940. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9086-3_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9086-3_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9085-6

  • Online ISBN: 978-1-4939-9086-3

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