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Patch-Clamp Recordings from Human Embryonic Stem Cells-Derived Fragile X Neurons

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Fragile-X Syndrome

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1942))

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Abstract

Performing electrophysiological recordings from human neurons that have been differentiated in vitro, as compared to primary cultures, raises many challenges. However, patch-clamp recording from neurons derived from stem cells provides an abundance of valuable information, reliably and fast. Here, we describe a protocol that is used successfully in our lab for recording from both control and Fragile X neurons, derived in vitro from human embryonic stem cells.

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References

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Author information

Authors and Affiliations

  1. Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA

    Michael Telias

  2. Department of Neurobiology, The Weizmann Institute of Science, Rehovot, Israel

    Menahem Segal

Authors
  1. Michael Telias
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  2. Menahem Segal
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Corresponding author

Correspondence to Michael Telias .

Editor information

Editors and Affiliations

  1. Wolfe PGD Stem Cell Lab, Racine IVF Unit at Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel

    Dalit Ben-Yosef

  2. Wolfe PGD Stem Cell Lab, Racine IVF Unit at Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel

    Yoav Mayshar

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Telias, M., Segal, M. (2019). Patch-Clamp Recordings from Human Embryonic Stem Cells-Derived Fragile X Neurons. In: Ben-Yosef, D., Mayshar, Y. (eds) Fragile-X Syndrome. Methods in Molecular Biology, vol 1942. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9080-1_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9080-1_11

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9079-5

  • Online ISBN: 978-1-4939-9080-1

  • eBook Packages: Springer Protocols

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