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Pre-Clinical Models

Techniques and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Paul C. Guest

    1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1916)

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About this book

This volume details reviews and protocols on the development and analysis of both cellular and animal-based pre-clinical models in a number of medical areas, including metabolic disorders, longevity, cancer, heart disease and psychiatric disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Pre-Clinical Models: Techniques and Protocols aims to provide methods that describe the context of specific disease or therapeutic areas.

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Reviews

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. A Guide to Mass Spectrometry-Based Quantitative Proteomics

      • Bradley J. Smith, Daniel Martins-de-Souza, Mariana Fioramonte
      Pages 3-39

    3. Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic, and Environmental Factors

      • Keiko Iwata
      Pages 41-48

    4. Monosodium Glutamate (MSG)-Induced Animal Model of Type 2 Diabetes

      • Zahra Bahadoran, Parvin Mirmiran, Asghar Ghasemi
      Pages 49-65

  3. Protocols

    1. Front Matter

      Pages 67-67
      Download chapter PDF

    2. The Elevated Plus Maze Test for Measuring Anxiety-Like Behavior in Rodents

      • Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai
      Pages 69-74

    3. The Forced Swim Test for Depression-Like Behavior in Rodents

      • Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai
      Pages 75-80

    4. Object Burying Test for Assessment of Obsessive Compulsive Behaviors in Mice

      • Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai
      Pages 81-85

    5. The Nest Building Test in Mice for Assessment of General Well-Being

      • Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai
      Pages 87-91

    6. Free Dyadic Social Interaction Test in Mice

      • Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai
      Pages 93-97

    7. The Open Field Test for Measuring Locomotor Activity and Anxiety-Like Behavior

      • Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai
      Pages 99-103

    8. The Y-Maze for Assessment of Spatial Working and Reference Memory in Mice

      • Ann-Katrin Kraeuter, Paul C. Guest, Zoltán Sarnyai
      Pages 105-111

    9. Maturation of a Human Oligodendrocyte Cell Line

      • Gabriela Seabra, Valéria de Almeida, Daniel Martins-de-Souza
      Pages 113-121

    10. Monitoring Age-Related Changes in the Lactate/Pyruvate Ratio Using a Colorimetric Assay in a C. elegans Model of Increased Life Span

      • Sumino Yanase, Kayo Yasuda, Naoki Ishii
      Pages 123-132

    11. Isolation and Characterization of Different Mesenchymal Stem Cell Populations from Rat Femur

      • Frans Alexander Jacobs, Mari van de Vyver, William Frank Ferris
      Pages 133-147

    12. Characterization of an Animal Model of Autism and Social Interaction

      • Keiko Iwata
      Pages 149-155

    13. Assaying Reproductive Capacity in Female Rodents

      • Catherine E. Aiken, Jane L. Tarry-Adkins, Susan E. Ozanne
      Pages 157-166

    14. Experimental Model of HindLimb Suspension-Induced Skeletal Muscle Atrophy in Rodents

      • Gabriel Nasri Marzuca-Nassr, Kaio Fernando Vitzel, Gilson Masahiro Murata, José Luis Márquez, Rui Curi
      Pages 167-176

    15. An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes Using Flow Cytometry

      • Yuji Takeda, Hironobu Asao, Ichiro Wakabayashi
      Pages 177-193
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Editors and Affiliations

  • Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

Bibliographic Information

  • Book Title: Pre-Clinical Models

  • Book Subtitle: Techniques and Protocols

  • Editors: Paul C. Guest

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8994-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8993-5Published: 11 December 2018

  • eBook ISBN: 978-1-4939-8994-2Published: 11 December 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 343

  • Number of Illustrations: 17 b/w illustrations, 73 illustrations in colour

  • Topics: Pharmacology/Toxicology, Animal Models

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