Abstract
DNA methylation is an important biomarker for gene activity. It contributes to gene silencing and is involved in regulating various seed developmental processes in plants. Many of these processes are involved in important traits associated with aspects of grain quality. A reliable, fast, and cheap method is the estimation of DNA methylation utilizing methylation sensitive restriction enzymes (MSRE) and quantitative real-time PCR (qPCR) for selected candidate regions. The presented method can be used to confirm an effect of RNAi constructs on their target genes or trans-activity. Analysis of promoter regions can contribute to estimation of gene activity and related traits.
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1 Electronic Supplementary Material
Supplementary Table 1
List of Enzymes with information on 5mC methylation sensitivity REBASE 2015 (http://rebase.neb.com/cgi-bin/mslist) (DOCX 59 KB)
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Seiler, C., Kuhlmann, M. (2019). Quantification of DNA Methylation as Biomarker for Grain Quality. In: Sreenivasulu, N. (eds) Rice Grain Quality. Methods in Molecular Biology, vol 1892. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8914-0_17
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DOI: https://doi.org/10.1007/978-1-4939-8914-0_17
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