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Autophagy

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Nicholas Ktistakis

    1. Signalling Programme, Babraham Institute, Cambridge, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Oliver Florey

    1. Signalling Programme, Babraham Institute, Cambridge, UK

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1880)

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About this book

This volume details a comprehensive and extensive set of protocols for the study of autophagy in vitro and in vivo. Chapters focus on mammals, various model organisms, and provide protocols for the study of autophagy-related processes outside of the canonical autophagy pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Autophagy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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Keywords

Table of contents (48 protocols)

  1. Front Matter

    Pages i-xvii
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  2. Autophagry In Vitro

    1. Front Matter

      Pages 1-1
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    2. Recombinant Expression, Purification, and Assembly of p62 Filaments

      • Abul K. Tarafder, Audrey Guesdon, Tanja Kuhm, Carsten Sachse
      Pages 3-15

    3. Structural Studies of Autophagy-Related Proteins

      • Melanie Schwarten, Oliver H. Weiergräber, Dušan Petrović, Birgit Strodel, Dieter Willbold
      Pages 17-56

    4. Structural Studies of Mammalian Autophagy Lipidation Complex

      • Kazuto Ohashi, Chinatsu Otomo, Zoltan Metlagel, Takanori Otomo
      Pages 57-75

    5. Structural Studies of Selective Autophagy in Yeast

      • Akinori Yamasaki, Yasunori Watanabe, Nobuo N. Noda
      Pages 77-90

    6. Biophysical Studies of LC3 Family Proteins

      • Javier H. Hervás, Zuriñe Antón, Alicia Alonso
      Pages 91-117

    7. Reconstituting Autophagy Initiation from Purified Components

      • Peter Mayrhofer, Thomas Wollert
      Pages 119-133

    8. Cell-Free Reconstitution of Autophagic Membrane Formation

      • Min Zhang, Liang Ge
      Pages 135-148

    9. Use of Peptide Arrays for Identification and Characterization of LIR Motifs

      • Mads Skytte Rasmussen, Åsa Birna Birgisdottir, Terje Johansen
      Pages 149-161

    10. Studying Autophagic Lysosome Reformation in Cells and by an In Vitro Reconstitution System

      • Yang Chen, Qian Peter Su, Li Yu
      Pages 163-172

    11. Formation of Autophagosomes Coincides with Relaxation of Membrane Curvature

      • Jaime Agudo-Canalejo, Roland L. Knorr
      Pages 173-188

    12. Studies of Receptor-Atg8 Interactions During Selective Autophagy

      • Christine Abert, Sascha Martens
      Pages 189-196

  3. Imaging Autophagy in Tissue Culture

    1. Front Matter

      Pages 197-197
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    2. Correlative Light and Electron Microscopy of Autophagosomes

      • Sigurdur Gudmundsson, Jenny Kahlhofer, Nastassia Baylac, Katri Kallio, Eeva-Liisa Eskelinen
      Pages 199-209

    3. Improved Electron Microscopy Fixation Methods for Tracking Autophagy-Associated Membranes in Cultured Mammalian Cells

      • Ritsuko Arai, Satoshi Waguri
      Pages 211-221

    4. Three-Color Simultaneous Live Imaging of Autophagy-Related Structures

      • Hiroyuki Ueda, Ouin Kunitaki, Maho Hamasaki
      Pages 223-230

    5. Correlative Live-Cell Imaging and Super-Resolution Microscopy of Autophagy

      • Eleftherios Karanasios
      Pages 231-242

    6. Methods for Imaging Autophagosome Dynamics in Primary Neurons

      • Audrey Dong, Vineet Vinay Kulkarni, Sandra Maday
      Pages 243-256

    7. Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson’s Disease Research

      • Petros Stathakos, Natalia Jimenez-Moreno, Lucy Crompton, Paul Nistor, Maeve A. Caldwell, Jon D. Lane
      Pages 257-280
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Editors and Affiliations

  • Signalling Programme, Babraham Institute, Cambridge, UK

    Nicholas Ktistakis, Oliver Florey

Bibliographic Information

  • Book Title: Autophagy

  • Book Subtitle: Methods and Protocols

  • Editors: Nicholas Ktistakis, Oliver Florey

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8873-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8872-3Published: 05 January 2019

  • eBook ISBN: 978-1-4939-8873-0Published: 04 January 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 741

  • Number of Illustrations: 38 b/w illustrations, 99 illustrations in colour

  • Topics: Biochemistry, general

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