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Fluorescence-Based Measurements of the CRAC Channel Activity in Cell Populations

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The CRAC Channel

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1843))

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Abstract

Cytosolic Ca2+ plays an important role in cellular biology, and since its identification as a second messenger, a number of techniques and methods to analyze the changes in cytosolic Ca2+ concentration ([Ca2+]c) induced by physiological agonists have been developed. Changes in [Ca2+]c might be determined in single cells or in cell populations. Measurement in single cells allows to determine changes in [Ca2+]c at a subcellular level but often results in heterogeneous responses among cells. Determination of intracellular Ca2+ mobilization at the cell population level reduces this heterogeneity and allows [Ca2+]c measurements in small cells that load little amounts of indicator. Here, we describe the measurement of agonist-evoked changes in [Ca2+]c associated with Ca2+ influx in cell populations.

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Acknowledgments

This work had been supported by Ministerio de Economia y Competitividad (BFU2013-45564-C2-1-P and BFU2016-74932-C2-1-P) and Junta de Extremadura-FEDER (GR15029 and IB16046). N.D. is supported by a fellowship from the University of Extremadura.

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Authors and Affiliations

  1. Department of Physiology (Cell Physiology Research Group), University of Extremadura, Cáceres, Spain

    Pedro C. Redondo, Alejandro Berna-Erro, Natalia Dionisio & Juan A. Rosado

Authors
  1. Pedro C. Redondo
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  2. Alejandro Berna-Erro
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  3. Natalia Dionisio
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  4. Juan A. Rosado
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Corresponding author

Correspondence to Juan A. Rosado .

Editor information

Editors and Affiliations

  1. IRSET Inserm UMR 1085/ UR1, Rennes, France

    Aubin Penna

  2. CNRS ERL 7003, Laboratoire STIM, Poitiers, France

    Bruno Constantin

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Redondo, P.C., Berna-Erro, A., Dionisio, N., Rosado, J.A. (2018). Fluorescence-Based Measurements of the CRAC Channel Activity in Cell Populations. In: Penna, A., Constantin, B. (eds) The CRAC Channel. Methods in Molecular Biology, vol 1843. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8704-7_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8704-7_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8702-3

  • Online ISBN: 978-1-4939-8704-7

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