Abstract
The technique of surface-enhanced laser desorption/ionization time-of-flight SELDI-TOF mass spectrometry could be used to establish serum or plasma proteomic profiles in esophageal adenocarcinoma. The protein profiles in patients may be used to predict survival and monitor response to chemotherapy in patients with esophageal adenocarcinoma. Here, a protocol for sample preparation from esophageal adenocarcinoma, analysis of proteomic profiles, and collection of protein fractions for identification of significant peaks is presented.
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References
Cahill DJ (2001) Protein and antibody arrays and their medical applications. J Immunol Methods 250:81–91
Plebani M (2005) Proteomics: the next revolution in laboratory medicine? Clin Chim Acta 357:13–22
Colantonio DA, Chan DW (2005) The clinical application of proteomics. Clin Chim Acta 357:151–158
Hu L, Ye M, Jiang X, Feng S, Zhou H et al (2007) Advances in hyphenated analytical techniques for shotgun proteome and peptidome analysis—a review. Anal Chim Acta 598:193–204
Pastwa E, Somiari SB, Czyz M, Somiari R (2007) Proteomics in human cancer research. Proteomics Clin Appl 1:4–17
Soldes OS, Kuick RD, Thompson IA, Hughes SJ, Orriger MB, Lannettoni MD, Hanash SM, Beer DG (1999) Differential expression of Hsp27 in normal oesophagus, Barrett’s metaplasia and esophageal adenocarcinomas. Br J Cancer 79:595–603
Paweletz CP, Ornstein DK, Roth MJ, Bichsel VE, Gillespie JW, Calvert VS et al (2000) Loss of annexin 1 correlates with early onset of tumorigenesis in esophageal and prostate carcinoma. Cancer Res 60:6293–6297
Hayashida Y, Honda K, Osaka Y, Hara T, Umaki T, Tsuchida A et al (2005) Possible prediction of chemoradiosensitivity of esophageal cancer by serum protein profiling. Clin Cancer Res 11:8042–8047
Kelly P, Appleyard V, Murray K, Paulin F, Lamont D, Baker L et al (2010) Detection of esophageal cancer biomarkers by plasma proteomic profiling of human cell line xenografts in response to chemotherapy. Br J Cancer 103:232–238
Kelly P, Paulin F, Lamont D, Baker L, Clearly S, Exon D, Thompson A (2012) Pre-treatment plasma proteomic markers associated with survival in esophageal cancer. Br J Cancer 106:955–961
Hortin GL, Sviridov D, Anderson NL (2008) High-abundance polypeptides of the human plasma proteome comprising the top 4 logs of polypetide abundance. Clin Chem 54:1608–1616
Zolotarjova N, Martosella J, Nicol G, Bailey J, Boyes BE, Barrett WC (2005) Differences among techniques for high-abundance protein depletion. Proteomics 5:3304–3313
Granger J, Siddiqui J, Copeland S, Remick D (2005) Albumin depletion of human plasma also removes low abundance proteins including the cytokines. Proteomics 5:4713–4718
Pappin DJ, Hojrup P, Bleasby AJ (1993) Rapid identification of proteins by peptide-mass fingerprinting. Curr Biol 3:327–332
Mascot Server (2016) Matrix Science, London. http://www.matrixscience.com. Accessed 15 Jan 2017
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Kelly, P. (2018). Proteomic Protocol in Esophageal Adenocarcinoma. In: Lam, A. (eds) Esophageal Adenocarcinoma. Methods in Molecular Biology, vol 1756. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7734-5_24
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DOI: https://doi.org/10.1007/978-1-4939-7734-5_24
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7733-8
Online ISBN: 978-1-4939-7734-5
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