Abstract
Our body contains a wide variety of fatty acids that differ in chain length, the degree of unsaturation, and location of the double bonds. As the various fatty acids play distinct roles in health and disease, methods that can specifically determine the fatty acid profile are needed for fundamental and clinical studies. Here we describe a method for the separation and quantification of fatty acids ranging from 8 to 24 carbon chain lengths in blood samples using gas chromatography-mass spectrometry following derivatization using pentafluorobenzyl bromide. This method quantitatively monitors fatty acid composition in a manner that satisfies the requirements for comprehensiveness, sensitivity, and accuracy.
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Hoving, L.R., Heijink, M., van Harmelen, V., van Dijk, K.W., Giera, M. (2018). GC-MS Analysis of Medium- and Long-Chain Fatty Acids in Blood Samples. In: Giera, M. (eds) Clinical Metabolomics. Methods in Molecular Biology, vol 1730. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7592-1_18
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DOI: https://doi.org/10.1007/978-1-4939-7592-1_18
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7591-4
Online ISBN: 978-1-4939-7592-1
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