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Bacterial Chemosensing

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Michael D. Manson

    1. Department of Biology, Texas A&M University, College Station, USA

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  • Includes cutting-edge methods and protocols for the study of bacterial chemosensing
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1729)

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About this book

This volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as Escherichia coli and Salmonella typhimurium, while being also applicable to a wide spectrum of other species. Beginning with an introduction, the sections of the book explore methods for studying bacterial chemotaxis at the population and whole-cell levels, in vivo analysis of receptor function, cryo-EM methods for studying chemoreceptor structure, as well as intracellular movement of chemosensory proteins, high-throughput methods to screen for novel chemoeffectors, and chemical tools and computer simulations for analyzing chemotaxis. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Bacterial Chemosensing: Methods and Protocols provides an extensive repertoire of approaches that can be extended to understanding chemotaxis, in particular, and chemosensing, in general, in the context of the enormously varied lifestyles adopted in the larger bacterial world.

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Diversity of Bacterial Chemosensing

      • Michael D. Manson
      Pages 3-6

    3. Transmembrane Signal Transduction in Bacterial Chemosensing

      • Michael D. Manson
      Pages 7-19

  3. Methods for Studying Bacterial Chemotaxis at the Population and Whole-Cell Levels

    1. Front Matter

      Pages 21-21
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    2. Two Spatial Chemotaxis Assays: The Nutrient-Depleted Chemotaxis Assay and the Agarose-Plug-Bridge Assay

      • Victoria Korolik, Karen M. Ottemann
      Pages 23-31

    3. Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries

      • Benjamin A. Webb, Timofey D. Arapov, Birgit E. Scharf
      Pages 33-45

    4. A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-linear Gradients

      • Nitesh Sule, Daniel Penarete-Acosta, Derek L. Englert, Arul Jayaraman
      Pages 47-59

    5. Visualizing Chemoattraction of Planktonic Cells to a Biofilm

      • Sneha Jani
      Pages 61-69

    6. Labeling Bacterial Flagella with Fluorescent Dyes

      • Linda Turner, Howard C. Berg
      Pages 71-76

  4. In Vivo Analysis of Receptor Function

    1. Front Matter

      Pages 77-77
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    2. All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins

      • Peter Ames, John S. Parkinson
      Pages 79-85

    3. Mutational Analysis of Binding Protein–Chemoreceptor Interactions

      • Andrew L. Seely
      Pages 87-94

    4. In Vitro Assay for Measuring Receptor-Kinase Activity in the Bacillus subtilis Chemotaxis Pathway

      • Hanna E. Walukiewicz, George W. Ordal, Christopher V. Rao
      Pages 95-105

    5. FRET Analysis of the Chemotaxis Pathway Response

      • Anja Paulick, Victor Sourjik
      Pages 107-126

    6. Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readout

      • Run-Zhi Lai, John S. Parkinson
      Pages 127-135

    7. Analyzing Protein Domain Interactions in Chemoreceptors by In Vivo PEGylation

      • Kylie J. Watts, Mark S. Johnson
      Pages 137-145

    8. Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid–Aqueous Interface

      • Rahmi Yusuf, Robert J. Lawrence, Lucy V. Eke, Roger R. Draheim
      Pages 147-158

    9. Analyzing Chemoreceptor Interactions In Vivo with the Trifunctional Cross-Linker TMEA

      • Claudia A. Studdert, Diego A. Massazza
      Pages 159-170

  5. Cryo-EM Methods for Studying Chemoreceptor Structure

    1. Front Matter

      Pages 171-171
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Editors and Affiliations

  • Department of Biology, Texas A&M University, College Station, USA

    Michael D. Manson

Bibliographic Information

  • Book Title: Bacterial Chemosensing

  • Book Subtitle: Methods and Protocols

  • Editors: Michael D. Manson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7577-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7576-1Published: 11 February 2018

  • Softcover ISBN: 978-1-4939-8526-5Published: 06 June 2019

  • eBook ISBN: 978-1-4939-7577-8Published: 10 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 389

  • Number of Illustrations: 29 illustrations in colour

  • Topics: Microbiology

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