Abstract
Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia.
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Shields, B.J., Keniry, A., Blewitt, M.E., McCormack, M.P. (2018). Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation. In: Jenkins, B. (eds) Inflammation and Cancer. Methods in Molecular Biology, vol 1725. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7568-6_15
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DOI: https://doi.org/10.1007/978-1-4939-7568-6_15
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7567-9
Online ISBN: 978-1-4939-7568-6
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