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Oxygenation Imaging by Nuclear Magnetic Resonance Methods

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Preclinical MRI

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1718))

Abstract

Oxygen monitoring is a topic of exhaustive research due to its central role in many biological processes, from energy metabolism to gene regulation. The ability to monitor in vivo the physiological distribution and the dynamics of oxygen from subcellular to macroscopic levels is a prerequisite to better understand the mechanisms associated with both normal and disease states (cancer, neurodegeneration, stroke, etc.). This chapter focuses on magnetic resonance imaging (MRI) based techniques to assess oxygenation in vivo. The first methodology uses injected fluorinated agents to provide quantitative pO2 measurements with high precision and suitable spatial and temporal resolution for many applications. The second method exploits changes in endogenous contrasts, i.e., deoxyhemoglobin and oxygen molecules through measurements of T 2* and T 1, in response to an intervention to qualitatively evaluate hypoxia and its potential modulation.

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Acknowledgements

Method development and application supported in part by CPRIT RP140399, RP120670-03, P30 CA142543, and P41 EB015908.

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Authors and Affiliations

  1. Prognostic Imaging Research Laboratory, Department of Radiology, UT Southwestern Medical Center, Dallas, TX, USA

    Heling Zhou & Ralph P. Mason

  2. Departament de Bioquímica i Biologia Molecular, Unitat de Bioquímica de Biociències, Edifici Cs, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain

    Nuria Arias-Ramos

  3. Instituto de Investigaciones Biomédicas ‘Alberto Sols’ C.S.I.C./U.A.M., Madrid, Spain

    Pilar López-Larrubia & Sebastián Cerdán

  4. Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas, Universidad Miguel Hernández, San Juan de Alicante, Alicante, Spain

    Jesús Pacheco-Torres

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  1. Heling Zhou
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  2. Nuria Arias-Ramos
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  3. Pilar López-Larrubia
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  4. Ralph P. Mason
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  5. Sebastián Cerdán
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  6. Jesús Pacheco-Torres
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Correspondence to Jesús Pacheco-Torres .

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Editors and Affiliations

  1. BIONAND, Andalusian Centre for Nanomedicine and Biotechnology, Junta de Andalucía, Universidad de Málaga, Málaga, Spain

    María Luisa García Martín

  2. Instituto de Investigaciones Biomédicas “Alberto Sols”, CSIC/UAM, Madrid, Spain

    Pilar López Larrubia

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Zhou, H., Arias-Ramos, N., López-Larrubia, P., Mason, R.P., Cerdán, S., Pacheco-Torres, J. (2018). Oxygenation Imaging by Nuclear Magnetic Resonance Methods. In: García Martín, M., López Larrubia, P. (eds) Preclinical MRI. Methods in Molecular Biology, vol 1718. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7531-0_18

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  • DOI: https://doi.org/10.1007/978-1-4939-7531-0_18

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