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Innate Immune Activation

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Dominic De Nardo

    1. Inflammation Division, The Walter & Eliza Hall Institute of Medical Research, Parkville, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Christine M De Nardo

    1. Chemical Biology Division, The Walter & Eliza Hall Institute of Medical Research, Parkville, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1714)

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About this book

This volume presents standard approaches and the most recent technical advances used to study innate immune activation. Chapters detail the assessment of macrophage activation, measuring innate immune responses to bacterial viability, quantification of secreted proteins, reporter systems, protocols examining specific innate immune activation by TLRs, RLRs, cGAS, and inflammasomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Innate Immune Activation: Methods and Protocols aims to be a useful and informative reference tool for further study into this vital field.

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Emerging Concepts in Innate Immunity

    • Karin Pelka, Dominic De Nardo
    Pages 1-18

  3. Bioinformatic Assessment of Macrophage Activation by the Innate Immune System

    • Thomas Ulas, Joachim L. Schultze, Marc Beyer
    Pages 19-40

  4. Generation of Genetic Knockouts in Myeloid Cell Lines Using a Lentiviral CRISPR/Cas9 System

    • Paul J. Baker, Seth L. Masters
    Pages 41-55

  5. Modeling Primary Human Monocytes with the Trans–Differentiation Cell Line BLaER1

    • Moritz M. Gaidt, Francesca Rapino, Thomas Graf, Veit Hornung
    Pages 57-66

  6. Measurement of NF-κB Activation in TLR-Activated Macrophages

    • Orna Ernst, Sharat J. Vayttaden, Iain D. C. Fraser
    Pages 67-78

  7. Biochemical Isolation of the Myddosome from Murine Macrophages

    • Yunhao Tan, Jonathan C. Kagan
    Pages 79-95

  8. Generation of Innate Immune Reporter Cells Using Retroviral Transduction

    • Yamel Cardona Gloria, Eicke Latz, Dominic De Nardo
    Pages 97-117

  9. Examining Myddosome Formation by Luminescence-Based Mammalian Interactome Mapping (LUMIER)

    • Olaf-Oliver Wolz, Manfred Koegl, Alexander N. R. Weber
    Pages 119-130

  10. Inflammatory Caspases: Activation and Cleavage of Gasdermin-D In Vitro and During Pyroptosis

    • Yue Zhao, Jianjin Shi, Feng Shao
    Pages 131-148

  11. Detection of ASC Speck Formation by Flow Cytometry and Chemical Cross-linking

    • Florian Hoss, Verena Rolfes, Mariana R. Davanso, Tarcio T. Braga, Bernardo S. Franklin
    Pages 149-165

  12. Measuring Innate Immune Responses to Bacterial Viability

    • Julien Moretti, Nicolas Vabret, J. Magarian Blander
    Pages 167-190

  13. Methods to Study Cell Swelling-Induced Inflammasome Activation

    • Vincent Compan, Pablo Pelegrín
    Pages 191-197

  14. Detecting Release of Bacterial dsDNA into the Host Cytosol Using Fluorescence Microscopy

    • Roland Felix Dreier, José Carlos Santos, Petr Broz
    Pages 199-213

  15. Quantitative Proteomics of Secreted Proteins

    • Annika Frauenstein, Felix Meissner
    Pages 215-227

  16. Simultaneous Detection of Cellular Viability and Interleukin-1β Secretion from Single Cells by ELISpot

    • Stephanie A. Conos, Lisa M. Lindqvist, James E. Vince
    Pages 229-236

  17. Detection and Quantification of MAVS Aggregation via Confocal Microscopy

    • Tan A. Nguyen, Lachlan Whitehead, Ken C. Pang
    Pages 237-247

  18. Back Matter

    Pages 249-251
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Editors and Affiliations

  • Inflammation Division, The Walter & Eliza Hall Institute of Medical Research, Parkville, Australia

    Dominic De Nardo

  • Chemical Biology Division, The Walter & Eliza Hall Institute of Medical Research, Parkville, Australia

    Christine M De Nardo

Bibliographic Information

  • Book Title: Innate Immune Activation

  • Book Subtitle: Methods and Protocols

  • Editors: Dominic De Nardo, Christine M De Nardo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7519-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7518-1Published: 25 November 2017

  • Softcover ISBN: 978-1-4939-8508-1Published: 05 September 2018

  • eBook ISBN: 978-1-4939-7519-8Published: 24 November 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 251

  • Number of Illustrations: 14 b/w illustrations, 40 illustrations in colour

  • Topics: Immunology

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