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Study of Plasmid-Mediated Quinolone Resistance in Bacteria

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DNA Topoisomerases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1703))

Abstract

Plasmid-mediated quinolone resistance (PMQR) involves genes for proteins that protect the quinolone targets, an enzyme that inactivates certain quinolones as well as aminoglycosides, and pumps that efflux quinolones. Quinolone susceptibility is reduced by these mechanisms but not to the level of clinical resistance unless chromosomal mutations are also present. PCR primers and conditions for PMQR gene detection are described as well as how to establish a plasmid location.

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Author information

Authors and Affiliations

  1. Lahey Hospital and Medical Center, 41 Mall Road, Burlington, MA, 01805, USA

    George A. Jacoby

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  1. George A. Jacoby
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Correspondence to George A. Jacoby .

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Editors and Affiliations

  1. Département de microbiologie, infectiologie et immunologie, Université de Montréal, Montréal, Québec, Canada

    Marc Drolet

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Jacoby, G.A. (2018). Study of Plasmid-Mediated Quinolone Resistance in Bacteria. In: Drolet, M. (eds) DNA Topoisomerases. Methods in Molecular Biology, vol 1703. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7459-7_22

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  • DOI: https://doi.org/10.1007/978-1-4939-7459-7_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7458-0

  • Online ISBN: 978-1-4939-7459-7

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