Abstract
In skeletal muscle, DNA methylation contributes to the suppression of gene expression in several biological processes and diseases. A protocol for the detection of methylated cytosine was thus established based on methylation-sensitive enzymes, immunoprecipitation, and bisulfite conversion. DNA methylation analysis, with bisulfite conversion and sequencing, enables the quantification of methylation at each single base position. Here, we describe a basic method of bisulfite sequencing that can be used to analyze local DNA methylation status to confirm genome-wide DNA methylation analysis or correlation of gene expression regulatory mechanisms.
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Miyata, K., Naito, M., Miyata, T., Mokuda, S., Asahara, H. (2017). Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells. In: Ryall, J. (eds) Skeletal Muscle Development. Methods in Molecular Biology, vol 1668. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7283-8_1
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DOI: https://doi.org/10.1007/978-1-4939-7283-8_1
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7282-1
Online ISBN: 978-1-4939-7283-8
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