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Tissue Culture (Somatic Embryogenesis)-Induced Tnt1 Retrotransposon-Based Mutagenesis in Brachypodium distachyon

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Brachypodium Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1667))

Abstract

Brachypodium distachyon is a model grass species for economically important cereal crops. Efforts are in progress to develop useful functional genomic resources in Brachypodium. A tobacco retrotransposon, Tnt1, has been used successfully in recent past to generate insertional mutagenesis in several dicot plant species. Tnt1 retrotransposon replicates, transposes, and inserts at multiple random genomic locations in the plant genome. Transposition occurs only during somatic embryogenesis but not during seed transmission. We developed Brachypodium transgenic plants that can express the Tnt1 element. Here, we describe an efficient tissue culture-based approach to generate Tnt1 insertional mutant population using transgenic Brachypodium line expressing the Tnt1 retrotransposon.

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Acknowledgement

We thank Janie Gallaway and Colleen Elles for taking care of the plants in the greenhouse. This work was supported by the Noble Research Institute, LLC.

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Authors and Affiliations

  1. Noble Research Institute, LLC., 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA

    Upinder S. Gill, Juan C. Serrani-Yarce, Hee-Kyung Lee & Kirankumar S. Mysore

  2. Department of Biological Sciences, University of North Texas, Denton, TX, 76203, USA

    Juan C. Serrani-Yarce

Authors
  1. Upinder S. Gill
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  2. Juan C. Serrani-Yarce
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  3. Hee-Kyung Lee
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  4. Kirankumar S. Mysore
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Corresponding author

Correspondence to Kirankumar S. Mysore .

Editor information

Editors and Affiliations

  1. Department of Biodiversity and Molecular Ecology, Fondazione Edmund Mach IASMA, Trentino, Italy

    Gaurav Sablok

  2. Sabanci University, Istanbul, Turkey

    Hikmet Budak

  3. Climate Change Cluster (C3), University of Technology Sydney, Sydney, New South Wales, Australia

    Peter J. Ralph

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Gill, U.S., Serrani-Yarce, J.C., Lee, HK., Mysore, K.S. (2018). Tissue Culture (Somatic Embryogenesis)-Induced Tnt1 Retrotransposon-Based Mutagenesis in Brachypodium distachyon . In: Sablok, G., Budak, H., Ralph, P. (eds) Brachypodium Genomics. Methods in Molecular Biology, vol 1667. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7278-4_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7278-4_5

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7276-0

  • Online ISBN: 978-1-4939-7278-4

  • eBook Packages: Springer Protocols

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