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c-di-GMP Signaling pp 169–186Cite as

Using Light-Activated Enzymes for Modulating Intracellular c-di-GMP Levels in Bacteria

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1657))

Abstract

Signaling pathways involving second messenger c-di-GMP regulate various aspects of bacterial physiology and behavior. We describe the use of a red light-activated diguanylate cyclase (c-di-GMP synthase) and a blue light-activated c-di-GMP phosphodiesterase (hydrolase) for manipulating intracellular c-di-GMP levels in bacterial cells. We illustrate the application of these enzymes in regulating several c-di-GMP-dependent phenotypes, i.e., motility and biofilm phenotypes in E. coli and chemotactic behavior in the alphaproteobacterium Azospirillum brasilense. We expect these light-activated enzymes to be also useful in regulating c-di-GMP-dependent processes occurring at the fast timescale, for spatial control of bacterial populations, as well as for analyzing c-di-GMP-dependent phenomena at the single-cell level.

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Acknowledgments

This work was supported in part by National Science Foundation grants MCB1052575 (to MG) and MCB13330344 (to GA). LON was supported by the National Institutes of Health grant R25GM086761.

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Authors and Affiliations

  1. Department of Molecular Biology, University of Wyoming, 1000 E. University Ave, Laramie, WY, 82071, USA

    Min-Hyung Ryu, Anastasia Fomicheva & Mark Gomelsky

  2. Current address: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA

    Min-Hyung Ryu

  3. Department of Biochemistry, Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN, 37996, USA

    Lindsey O’Neal & Gladys Alexandre

Authors
  1. Min-Hyung Ryu
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  2. Anastasia Fomicheva
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  3. Lindsey O’Neal
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  4. Gladys Alexandre
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  5. Mark Gomelsky
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Corresponding author

Correspondence to Mark Gomelsky .

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Editors and Affiliations

  1. Department of Biological Sciences, Binghamton University Department of Biological Sciences, Binghamton, New York, USA

    Karin Sauer

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Ryu, MH., Fomicheva, A., O’Neal, L., Alexandre, G., Gomelsky, M. (2017). Using Light-Activated Enzymes for Modulating Intracellular c-di-GMP Levels in Bacteria. In: Sauer, K. (eds) c-di-GMP Signaling. Methods in Molecular Biology, vol 1657. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7240-1_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7240-1_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7239-5

  • Online ISBN: 978-1-4939-7240-1

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