Abstract
Hybridization chain reaction (HCR) provides a feasible solution for nucleic acid detection without target amplification. By highly specific sandwich hybridization, target RNA can be directly captured onto solid support and detected using HCR with fluorescent dyes. Here, we describe a novel method for malaria RNA detection based on sandwich hybridization and two-dimensional HCR, without involving nucleic acid purification or any enzymatic reaction, using ordinary oligonucleotides without labeling or modification.
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Xu, Y., Zheng, Z. (2018). Hybridization Chain Reaction for Direct mRNA Detection Without Nucleic Acid Purification. In: Gaspar, I. (eds) RNA Detection. Methods in Molecular Biology, vol 1649. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7213-5_12
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DOI: https://doi.org/10.1007/978-1-4939-7213-5_12
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7212-8
Online ISBN: 978-1-4939-7213-5
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