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Site-Specific Recombinases pp 1–19Cite as

Generating Genetically Modified Mice: A Decision Guide

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1642))

Abstract

The generation of a new genetically modified mouse strain is a big hurdle to take for many researchers. It is often unclear which steps and decisions have to be made prior to obtaining the desired mouse model. This review aims to help researchers by providing a decision guide that answers the essential questions that need to be asked before generating the most suitable genetically modified mouse line in the most optimal timeframe. The review includes the latest technologies in both the stem cell culture and gene editing tools, particularly CRISPR/Cas9, and provides compatibility guidelines for selecting among the different types of genetic modifications that can be introduced in the mouse genome and the various routes for introducing these modifications into the mouse germline.

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  1. Mouse Clinic for Cancer and Aging, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands

    Ivo J. Huijbers

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Correspondence to Ivo J. Huijbers .

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  1. Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA

    Nikolai Eroshenko

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Huijbers, I.J. (2017). Generating Genetically Modified Mice: A Decision Guide. In: Eroshenko, N. (eds) Site-Specific Recombinases. Methods in Molecular Biology, vol 1642. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7169-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7169-5_1

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