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Germinal Centers

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Dinis Pedro Calado

    1. Immunity and Cancer Laboratory, The Francis Crick Institute, and King’s College London, London, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1623)

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About this book

This volume provides key methods and protocols from laboratories engaged in germinal centers (GC) research with the expectation of stimulating further research, and to aid scientists in the study of GC biology and pathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Germinal Centers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Analysis of the Germinal Center Reaction in Tissue Sections

    • David Dominguez-Sola, Giorgio Cattoretti
    Pages 1-20

  3. Detecting Gene Expression in Lymphoid Microenvironments by Laser Microdissection and Quantitative RT-PCR

    • Yang Zhang, Laura Garcia-Ibanez, Geoffrey Brown, Kai-Michael Toellner
    Pages 21-36

  4. Tracking Plasma Cell Differentiation in Living Mice with Two-Photon Microscopy

    • Carolin Ulbricht, Randall L. Lindquist, Laura Tech, Anja E. Hauser
    Pages 37-50

  5. Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation

    • Johanne T. Jacobsen, Gabriel D. Victora
    Pages 51-58

  6. Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-Photon Photoconversion

    • Imogen Moran, Tri Giang Phan
    Pages 59-72

  7. Intravital Microscopy of T–B Cell Interactions in Germinal Centers

    • Changming Shih, Hai Qi
    Pages 73-85

  8. Identifying Follicular Regulatory T Cells by Confocal Microscopy

    • Ine Vanderleyden, Michelle A. Linterman
    Pages 87-93

  9. Cytokine Expression by T Follicular Helper Cells

    • Christoph Jandl, Claudia Loetsch, Cecile King
    Pages 95-103

  10. Follicular Dendritic Cell Isolation and Loading of Immune Complexes

    • Balthasar A. Heesters, Cees E. Van der Poel, Michael C. Carroll
    Pages 105-112

  11. Isolation and Characterization of Mouse and Human Follicular Dendritic Cells

    • Mohey Eldin M. El Shikh, Riham El Sayed, Costantino Pitzalis
    Pages 113-123

  12. In Vitro-Induced Germinal Center B Cell Culture System

    • Kei Haniuda, Takuya Nojima, Daisuke Kitamura
    Pages 125-133

  13. CRISPR/Cas9-Mediated In Vitro Mutagenesis in GC-Like B Cells

    • Van Trung Chu, Robin Graf, Klaus Rajewsky
    Pages 135-145

  14. Germinal Center Formation with Retrovirally Transduced B Cells for Determining the Role of Specific Molecules In Vivo

    • Rinako Nakagawa
    Pages 147-158

  15. Characterization of the B Cell Transcriptome Bound by RNA-Binding Proteins with iCLIP

    • Manuel D. Díaz-Muñoz, Elisa Monzón-Casanova, Martin Turner
    Pages 159-179

  16. Flow-Cytometric Method Measuring B Cell Surface Immunoglobulin Avidity

    • Davide Angeletti, Gregory M. Frank, Jonathan W. Yewdell
    Pages 181-189

  17. Somatic Hypermutation and Affinity Maturation Analysis Using the 4-Hydroxy-3-Nitrophenyl-Acetyl (NP) System

    • Nicole Heise, Ulf Klein
    Pages 191-208

  18. Targeting Gene Function in Germinal Center B Cells: A Practical Approach

    • Valentina Petrocelli, Stefano Casola
    Pages 209-231

  19. Development of Mouse Model Systems of Germinal Center Lymphomas

    • Eleni Kabrani, Sandrine Sander
    Pages 233-242

  20. The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas

    • Simon Le Gallou, Takuya Nojima, Daisuke Kitamura, Jean-Claude Weill, Claude-Agnès Reynaud
    Pages 243-251
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Editors and Affiliations

  • Immunity and Cancer Laboratory, The Francis Crick Institute, and King’s College London, London, United Kingdom

    Dinis Pedro Calado

Bibliographic Information

  • Book Title: Germinal Centers

  • Book Subtitle: Methods and Protocols

  • Editors: Dinis Pedro Calado

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7095-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7094-0Published: 08 June 2017

  • Softcover ISBN: 978-1-4939-8383-4Published: 02 August 2018

  • eBook ISBN: 978-1-4939-7095-7Published: 06 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 338

  • Number of Illustrations: 15 b/w illustrations, 49 illustrations in colour

  • Topics: Immunology

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