Abstract
Aptamer-based trapping techniques are in general suitable to replace common antibody-based enrichment approaches. A time-consuming isolation or clean-up is often necessary during sample preparation, e.g. for the detection of spores. For the development of bioanalytical routine approaches, aptamers with a high affinity to B. cereus spores were applied for the establishment and validation of an aptamer-based trapping technique in milk with fat contents between 0.3 and 3.5%. Thereby, enrichment factors of up to sixfold were achieved. The combination of an aptamer-based enrichment by magnetic separation and the subsequent specific real-time PCR detection represents a reliable and rapid detection system.
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Acknowledgement
This research project was supported by the German Ministry of Economics and Technology (via AiF) and the FEI (Forschungskreis der Ernaehrungsindustrie e. V., Bonn, Germany); Project AiF 331 ZN.
The author’s thank Dr. Tim Hünniger, Jan-Hinnerk Jarck, and Esther Frohnmeyer for practical support and discussion.
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Fischer, C., Fischer, M. (2017). Aptamer-Based Trapping: Enrichment of Bacillus cereus Spores for Real-Time PCR Detection. In: Holst, O. (eds) Microbial Toxins. Methods in Molecular Biology, vol 1600. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6958-6_6
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DOI: https://doi.org/10.1007/978-1-4939-6958-6_6
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