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Lysosomes

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Karin Öllinger

    1. Experimental Pathology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  2. Hanna Appelqvist

    1. Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1594)

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About this book

This volume provides laboratory protocols essential for studies on lysosomal biology. Chapters aim to guide researchers in their exploration of lysosomes, both under normal conditions and in pathological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Lysosome: Methods and Protocols aims to provided protocols that will guide and inspire further research and generate new insights into this fascinating organelle.

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Keywords

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS

    • Melanie Thelen, Dominic Winter, Thomas Braulke, Volkmar Gieselmann
    Pages 1-18

  3. Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics

    • Mesut Bilgin, Jesper Nylandsted, Marja Jäättelä, Kenji Maeda
    Pages 19-34

  4. Analysis of N- and O-Glycosylation of Lysosomal Glycoproteins

    • Elmira Tokhtaeva, Olga A. Mareninova, Anna S. Gukovskaya, Olga Vagin
    Pages 35-42

  5. Analyzing Lysosome-Related Organelles by Electron Microscopy

    • Ilse Hurbain, Maryse Romao, Ptissam Bergam, Xavier Heiligenstein, Graça Raposo
    Pages 43-71

  6. Microscopic Analysis of Lysosomal Membrane Permeabilization

    • Ana Maria Vilamill Giraldo, Karin Öllinger, Vesa Loitto
    Pages 73-92

  7. Quantitative Co-Localization and Pattern Analysis of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets

    • Frederik W. Lund, Daniel Wüstner
    Pages 93-128

  8. Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues

    • Mingguang Ren, Beibei Deng, Xiuqi Kong, Yonghe Tang, Weiying Lin
    Pages 129-139

  9. Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy-Dependent Recovery

    • Takanobu Otomo, Tamotsu Yoshimori
    Pages 141-149

  10. Delivery of Cargo to Lysosomes Using GNeosomes

    • Kristina M. Hamill, Ezequiel Wexselblatt, Wenyong Tong, Jeffrey D. Esko, Yitzhak Tor
    Pages 151-163

  11. Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles

    • Camilla Lööv, Anna Erlandsson
    Pages 165-177

  12. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells

    • Ida Eriksson, Karin Öllinger, Hanna Appelqvist
    Pages 179-189

  13. Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry

    • Anna L. Södergren, Sofia Ramström
    Pages 191-203

  14. Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes

    • Norma W. Andrews
    Pages 205-211

  15. Using the MEROPS Database for Investigation of Lysosomal Peptidases, Their Inhibitors, and Substrates

    • Neil D. Rawlings
    Pages 213-226

  16. Next-Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of LysoPlex

    • Giuseppina Di Fruscio, Sandro Banfi, Vincenzo Nigro, Andrea Ballabio
    Pages 227-241

  17. Gelatin Zymography Using Leupeptin for the Detection of Various Cathepsin L Forms

    • Yoko Hashimoto
    Pages 243-254

  18. Methods for Determination of α-Glycosidase, β-Glycosidase, and α-Galactosidase Activities in Dried Blood Spot Samples

    • Eser Yıldırım Sozmen, Ebru Demirel Sezer
    Pages 255-264

  19. Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi

    • Jyotsna Verma, Sunita Bijarnia-Mahay, Ishwar C. Verma
    Pages 265-291

  20. Lysosomal Biology in Cancer

    • Colin Fennelly, Ravi K. Amaravadi
    Pages 293-308
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Editors and Affiliations

  • Experimental Pathology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden

    Karin Öllinger

  • Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden

    Hanna Appelqvist

Bibliographic Information

  • Book Title: Lysosomes

  • Book Subtitle: Methods and Protocols

  • Editors: Karin Öllinger, Hanna Appelqvist

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6934-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6932-6Published: 30 April 2017

  • Softcover ISBN: 978-1-4939-8343-8Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6934-0Published: 29 April 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 311

  • Number of Illustrations: 17 b/w illustrations, 61 illustrations in colour

  • Topics: Cell Biology

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