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Studying the Dynamics of TCR Internalization at the Immune Synapse

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The Immune Synapse

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1584))

Abstract

Establishing a stable interaction between a T cell and an antigen presenting cell (APC) involves the formation of an immune synapse (IS). It is through this structure that the T cell can integrate all the signals provided by the APC. The IS also serves as a mechanism for TCR downregulation through internalization. Here, we describe methods for visualizing MHC-engaged T cell receptor (TCR) internalization from the IS in human cell lines and mouse primary T cells by confocal fluorescence microscopy techniques.

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Author information

Authors and Affiliations

  1. Cell Biology and Immunology Department, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Madrid, Spain

    Enrique Calleja, Balbino Alarcón & Clara L. Oeste

Authors
  1. Enrique Calleja
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  2. Balbino Alarcón
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  3. Clara L. Oeste
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Corresponding author

Correspondence to Clara L. Oeste .

Editor information

Editors and Affiliations

  1. Department of Life sciences, University of Siena, Siena, Siena, Italy

    Cosima T. Baldari

  2. University of Oxford, Kennedy Institute of Rheumatology, Headington, Oxford, United Kingdom

    Michael L. Dustin

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Calleja, E., Alarcón, B., Oeste, C.L. (2017). Studying the Dynamics of TCR Internalization at the Immune Synapse. In: Baldari, C., Dustin, M. (eds) The Immune Synapse. Methods in Molecular Biology, vol 1584. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6881-7_7

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  • DOI: https://doi.org/10.1007/978-1-4939-6881-7_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6879-4

  • Online ISBN: 978-1-4939-6881-7

  • eBook Packages: Springer Protocols

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