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NanoScript: A Versatile Nanoparticle-Based Synthetic Transcription Factor for Innovative Gene Manipulation

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Biomedical Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1570))

Abstract

Cellular reprogramming and stem cell-based therapies have shown tremendous potential in the field of regenerative medicine. To that end, developing tools to control stem cell fate is an attractive area of research for replacing damaged and diseased cells and reestablishing functional connections for tissue repair. Transcription factor (TFs) proteins are well known to regulate gene expression and direct stem cell fate. Inspired by natural TFs, NanoScript, a nanoparticle (NP)-based platform, mimics TFs to afford control over gene expression and stem cell fate for regenerative medicine. Here, we describe the construction of the NanoScript platform, which is designed with tunable properties to replicate the structure and function of TFs to bind to specific portions of the genome and regulate gene expression in a way that does not involve viral delivery.

The original version of this chapter was revised. An erratum to the chapter can be found at DOI: 10.1007/978-1-4939-6840-4_23

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-6840-4_23

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Authors and Affiliations

  1. Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ, 08854, USA

    Kholud Dardir, Christopher Rathnam & Ki-Bum Lee

Authors
  1. Kholud Dardir
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  2. Christopher Rathnam
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  3. Ki-Bum Lee
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Corresponding author

Correspondence to Ki-Bum Lee .

Editor information

Editors and Affiliations

  1. Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, Illinois, USA

    Sarah Hurst Petrosko

  2. Department of Biomedical Engineering, University of Delaware, Newark, Delaware, USA

    Emily S. Day

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Dardir, K., Rathnam, C., Lee, KB. (2017). NanoScript: A Versatile Nanoparticle-Based Synthetic Transcription Factor for Innovative Gene Manipulation. In: Petrosko, S., Day, E. (eds) Biomedical Nanotechnology. Methods in Molecular Biology, vol 1570. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6840-4_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6840-4_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6838-1

  • Online ISBN: 978-1-4939-6840-4

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