Abstract
Annonaceous acetogenins (ACGs) are molecules with carbon numbers C35–C37, usually with tetrahydrofuran and tetrahydropyran rings and one terminal γ-lactone (usually α,β-unsaturated), in a large aliphatic chain that is varyingly hydroxylated, acetoxylated or ketonized. ACGs have ecological functions as insecticides and are pharmacologically promising due to their cytotoxic and antitumoral properties. They are found in the seeds, leaves, roots, flowers and fruits of annonaceous plants and can be detected during isolation via thin-layer chromatography using Kedde’s reagent, which reacts with the unsaturated lactone. This chapter describes the location in situ of ACGs in fresh sections of annonaceous seeds using Kedde’s reagent.
The acetogenins are located in the idioblasts, in the endosperm and in the embryonic axis during differentiation. This method can aid in the detection of ACGs with a terminal unsaturated γ-lactone in organs and tissues.
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Acknowledgments
The authors thank Dr. Patricia Rivas Manzano and Biol. Carlos Tonatiuh Chavira Ramírez for technical assistance with the cryostat sectioning in the Laboratory of Tissue and Reproductive Biology, Faculty of Sciences, National Autonomous University of Mexico.
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Laguna-Hernández, G., Brechú-Franco, A.E., De la Cruz-Chacón, I., González-Esquinca, A.R. (2017). A Histochemical Technique for the Detection of Annonaceous Acetogenins. In: Pellicciari, C., Biggiogera, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 1560. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6788-9_25
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DOI: https://doi.org/10.1007/978-1-4939-6788-9_25
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