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Visualization of the T Cell Response in Contact Hypersensitivity

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Book cover Inflammation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1559))

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Abstract

Contact hypersensitivity (CHS) is the most basic murine model for type IV hypersensitivity. This dermatitis model is mediated by hapten-sensitized, skin-infiltrating T cells. Recent intravital imaging studies have demonstrated the dynamic migratory property of skin-infiltrating T cells and the orchestrated interaction of T cells with dermal dendritic cells. Multiphoton microscopy enables the direct, three-dimensional, and minimally invasive imaging of in vivo skin samples with high spatiotemporal resolution. Here, we describe a basic method for the intravital imaging of skin-infiltrating T cells in the DNFB-induced CHS response.

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Acknowledgment

This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Dermatology, Kyoto University Graduate School of Medicine, 54 Shogoin-Kawahara, Kyoto, 606-8507, Japan

    Gyohei Egawa M.D., Ph.D. & Kenji Kabashima

Authors
  1. Gyohei Egawa M.D., Ph.D.
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  2. Kenji Kabashima
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Corresponding author

Correspondence to Gyohei Egawa M.D., Ph.D. .

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Editors and Affiliations

  1. Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany

    Björn E. Clausen

  2. Section Medical Physiology Department of Neuroscience, University of Groningen University Medical Center Groningen, Groningen, The Netherlands

    Jon D. Laman

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Egawa, G., Kabashima, K. (2017). Visualization of the T Cell Response in Contact Hypersensitivity. In: Clausen, B., Laman, J. (eds) Inflammation. Methods in Molecular Biology, vol 1559. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6786-5_4

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  • DOI: https://doi.org/10.1007/978-1-4939-6786-5_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6784-1

  • Online ISBN: 978-1-4939-6786-5

  • eBook Packages: Springer Protocols

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