Abstract
Histochemical assays of xylem cell death cannot take advantage of the conventional methods for detection of cell death, such as staining with propidium iodide or trypan/Evans blue or the TUNEL staining. This chapter presents two alternative histochemical methods that can be used to detect xylem cell death quickly and reliably using light microscopy. The first method is a viability stain that can be used to detect cell death of different types of xylem elements in basically any plant species. The second method reveals cell death in xylem vessel elements based on their functionality in transport of water and small water-soluble stains.
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Acknowledgment
This work was supported by grants 232-2009-1698 and 232-2011-1312 from the Swedish Research Council Formas, and grant 621-2013-4949 from the Swedish Research Council VR. We wish to acknowledge the Plant Cell Wall Laboratory at Umeå Plant Science Centre.
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Escamez, S., Bollhöner, B., Tuominen, H. (2017). Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues. In: de Lucas, M., Etchhells, J. (eds) Xylem. Methods in Molecular Biology, vol 1544. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6722-3_3
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DOI: https://doi.org/10.1007/978-1-4939-6722-3_3
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