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Hepatitis B Virus pp 259–266Cite as

Partial Hepatectomy and Castration of HBV Transgenic Mice

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1540))

Abstract

Hepatitis B virus (HBV) is a hepatotropic virus. Its infection can cause liver injury and regeneration, and its replication is affected by the gender. Transgenic mice that carry a 1.3-mer overlength HBV DNA genome productively replicate HBV in hepatocytes and have been very useful for studying the replication and pathogenesis of HBV in vivo. By using this mouse model, the relationship between HBV and liver injury and regeneration as well as the effect of the gender on HBV gene expression and replication has been studied. In this chapter, we describe the surgical procedures of partial hepatectomy and castration and provide examples to demonstrate how these surgical procedures may be used to study the effect of HBV on liver regeneration and the effect of androgen on HBV replication.

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References

  1. Yu MW, Yang YC, Yang SY, Cheng SW, Liaw YF, Lin SM, Chen CJ (2001) Hormonal markers and hepatitis B virus-related hepatocellular carcinoma risk: a nested case-control study among men. J Natl Cancer Inst 93(21):1644–1651

    Article  CAS  PubMed  Google Scholar 

  2. Fausto N, Campbell JS, Riehle KJ (2006) Liver regeneration. Hepatology 43(2 Suppl 1):S45–S53

    Article  CAS  PubMed  Google Scholar 

  3. Chisari FV (1995) Hepatitis B virus transgenic mice: insights into the virus and the disease. Hepatology 22(4 Pt 1):1316–1325

    CAS  PubMed  Google Scholar 

  4. Chisari FV (1996) Hepatitis B virus transgenic mice: models of viral immunobiology and pathogenesis. Curr Top Microbiol Immunol 206:149–173

    CAS  PubMed  Google Scholar 

  5. Tian Y, Kuo CF, Chen WL, Ou JH (2012) Enhancement of hepatitis B virus replication by androgen and its receptor in mice. J Virol 86(4):1904–1910

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Higgins GMA, Anderson RM (1931) Experimental pathology of the liver: I. Restoration of the liver of the white rat following partial surgical removal. Arch Pathol 12:186–202

    Google Scholar 

  7. Mitchell C, Willenbring H (2008) A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice. Nat Protoc 3(7):1167–1170

    Article  CAS  PubMed  Google Scholar 

  8. Nikfarjam M, Malcontenti-Wilson C, Fanartzis M, Daruwalla J, Christophi C (2004) A model of partial hepatectomy in mice. J Invest Surg 17(5):291–294

    Article  PubMed  Google Scholar 

  9. Inderbitzin D, Studer P, Sidler D, Beldi G, Djonov V, Keogh A, Candinas D (2006) Regenerative capacity of individual liver lobes in the microsurgical mouse model. Microsurgery 26(6):465–469

    Article  PubMed  Google Scholar 

  10. Greene AK, Puder M (2003) Partial hepatectomy in the mouse: technique and perioperative management. J Invest Surg 16(2):99–102

    Article  PubMed  Google Scholar 

  11. Hori T, Ohashi N, Chen F, Baine AM, Gardner LB, Jermanus S, Nguyen JH (2011) Simple and sure methodology for massive hepatectomy in the mouse. Ann Gastroenterol 24(4):307–318

    PubMed  PubMed Central  Google Scholar 

  12. Hori T, Ohashi N, Chen F, Baine AM, Gardner LB, Hata T, Uemoto S, Nguyen JH (2012) Simple and reproducible hepatectomy in the mouse using the clip technique. World J Gastroenterol 18(22):2767–2774

    Article  PubMed  PubMed Central  Google Scholar 

  13. Dervan PA, Magee HM, Buckley C, Carney DN (1992) Proliferating cell nuclear antigen counts in formalin-fixed paraffin-embedded tissue correlate with Ki-67 in fresh tissue. Am J Clin Pathol 97(5 Suppl 1):S21–S28

    CAS  PubMed  Google Scholar 

  14. Mancini R, Marucci L, Benedetti A, Jezequel AM, Orlandi F (1994) Immunohistochemical analysis of S-phase cells in normal human and rat liver by PC10 monoclonal antibody. Liver 14(2):57–64

    Article  CAS  PubMed  Google Scholar 

  15. Hall PA, Levison DA, Woods AL, Yu CC, Kellock DB, Watkins JA, Barnes DM, Gillett CE, Camplejohn R, Dover R et al (1990) Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162(4):285–294

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, Los Angeles, CA, USA

    Yongjun Tian & Jing-hsiung James Ou

Authors
  1. Yongjun Tian
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  2. Jing-hsiung James Ou
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Corresponding author

Correspondence to Jing-hsiung James Ou .

Editor information

Editors and Affiliations

  1. Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA

    Haitao Guo

  2. Arbutus Biopharma, Inc., Doylestown, Pennsylvania, USA

    Andrea Cuconati

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Tian, Y., Ou, Jh.J. (2017). Partial Hepatectomy and Castration of HBV Transgenic Mice. In: Guo, H., Cuconati, A. (eds) Hepatitis B Virus. Methods in Molecular Biology, vol 1540. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6700-1_22

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  • DOI: https://doi.org/10.1007/978-1-4939-6700-1_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6698-1

  • Online ISBN: 978-1-4939-6700-1

  • eBook Packages: Springer Protocols

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