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Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains Using High Throughput Sequencing

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Antibiotics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1520))

Abstract

High-Throughput Sequencing (HTS) technologies transformed the microbial typing and molecular epidemiology field by providing the cost-effective ability for researchers to probe draft genomes, not only for epidemiological markers but also for antibiotic resistance and virulence determinants. In this chapter, we provide protocols for the analysis of HTS data for the determination of multilocus sequence typing (MLST) information and for determining presence or absence of antibiotic resistance genes.

The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-6634-9_21

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-6634-9_21

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Author information

Authors and Affiliations

  1. Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal

    Miguel Paulo Machado, Bruno Ribeiro-Gonçalves, Mickael Silva, Mário Ramirez & João André Carriço

Authors
  1. Miguel Paulo Machado
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  2. Bruno Ribeiro-Gonçalves
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  3. Mickael Silva
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  4. Mário Ramirez
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  5. João André Carriço
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Corresponding author

Correspondence to João André Carriço .

Editor information

Editors and Affiliations

  1. Interfaculty Institute for Microbiology and Infection Medicine, Microbial Bioactive Compounds, University of Tübingen, Tübingen, Germany

    Peter Sass

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Machado, M.P., Ribeiro-Gonçalves, B., Silva, M., Ramirez, M., Carriço, J.A. (2017). Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains Using High Throughput Sequencing. In: Sass, P. (eds) Antibiotics. Methods in Molecular Biology, vol 1520. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6634-9_20

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  • DOI: https://doi.org/10.1007/978-1-4939-6634-9_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6632-5

  • Online ISBN: 978-1-4939-6634-9

  • eBook Packages: Springer Protocols

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