Abstract
Pin1 knockout in mice causes age-dependent neuropathy characterized by motor and behavioral deficits, tau hyper phosphorylation, tau filament formation, and neuronal degradation. Here, we describe the methods with mouse behavior test, immunostaining, and immunoblotting to detect many aspects of neurodegeneration in Pin1 knockout mice.
Asami Kondo and Onder Albayram have contributed equally to this work.
Xiao Zhen Zhou and Kun Ping Lu have contributed equally to this work.
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Acknowledgments
This work is supported by NIH grants R01CA167677, R03DA031663, R01HL111430, R01AG029385, and R01AG046319, and Alzheimer’s Association grant DVT-14-322623 to K.P.L., and BIDMC pilot grant and gift donations from the Owens Family Foundation to X.Z.Z. and K.P.L.
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Kondo, A., Albayram, O., Zhou, X.Z., Lu, K.P. (2017). Pin1 Knockout Mice: A Model for the Study of Tau Pathology in Alzheimer’s Disease. In: Smet-Nocca, C. (eds) Tau Protein. Methods in Molecular Biology, vol 1523. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6598-4_28
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DOI: https://doi.org/10.1007/978-1-4939-6598-4_28
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