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Drug Target miRNA

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Marco F. Schmidt

    1. BEROCEUTICA GmbH, Potsdam, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1517)

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About this book

This volume provides a concise and technical discussion of recently developed approaches to overcome challenges, such as pharmacodynamics and pharmacokinetics difficulties, in miRNA drug discovery. These strategies cover anti-sense agents targeting miRNA that are applied in advanced formulations or are chemically optimized to increase delivery; small molecule miRNA modulators to overcome anti-sense agents’ limitations; general enhancers of miRNA maturation; and Argonaute 2 protein and its pharmacokinetic parameters. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Drug Target miRNA: Methods and Protocols is a valuable resource for anyone interested in the ever-evolving field of miRNA drug discovery.

 

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Reviews

    1. Front Matter

      Pages 1-1
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    2. miRNA Targeting Drugs: The Next Blockbusters?

      • Marco F. Schmidt
      Pages 3-22

  3. Protocols

    1. Front Matter

      Pages 23-23
      Download chapter PDF

    2. Functional Analysis of miRNAs Using the DIANA Tools Online Suite

      • Ioannis S. Vlachos, Artemis G. Hatzigeorgiou
      Pages 25-50

    3. Non-nucleotide Modification of Anti-miRNA Oligonucleotides

      • Kim A. Lennox, Christopher A. Vakulskas, Mark A. Behlke
      Pages 51-69

    4. Quantification of Oligonucleotide Association with miRNA–Argonaute Complexes In Vitro

      • Dimitrios G. Zisoulis
      Pages 71-78

    5. Determination of Anti-miR Association with miRNA/Argonaute Complexes In Vivo

      • Dimitrios G. Zisoulis
      Pages 79-89

    6. Competitive Argonaute-Based RNA Immunoprecipitation for Investigation of Transcriptomic Response to Anti-miR

      • John R. Androsavich
      Pages 91-102

    7. Assessing Anti-miR Pharmacology with miRNA Polysome Shift Assay

      • John R. Androsavich
      Pages 103-113

    8. Evaluating Synergistic Effects of miR-34a Mimics in Combination with Other Therapeutic Agents in Cultured Non-Small Cell Lung Cancer Cells

      • Jane Zhao, Andreas G. Bader
      Pages 115-126

    9. Assessing the Off-Target Effects of miRNA Inhibitors on Innate Immune Toll-Like Receptors

      • Geneviève Pépin, Jonathan Ferrand, Michael P. Gantier
      Pages 127-135

    10. Design of Multimodal Small Molecules Targeting miRNAs Biogenesis: Synthesis and In Vitro Evaluation

      • Duc D. Vo, Maria Duca
      Pages 137-154

    11. Machine Learning Approaches Toward Building Predictive Models for Small Molecule Modulators of miRNA and Its Utility in Virtual Screening of Molecular Databases

      • Vinita Periwal, Vinod Scaria
      Pages 155-168

    12. Identification of Small Molecule Modulators of MicroRNA by Library Screening

      • Zhangang Xiao, Yangchao Chen
      Pages 169-178

    13. Rapid Generation of miRNA Inhibitor Leads by Bioinformatics and Efficient High-Throughput Screening Methods

      • Christopher L. Haga, Sai Pradeep Velagapudi, Jessica L. Childs-Disney, Jacqueline Strivelli, Matthew D. Disney, Donald G. Phinney
      Pages 179-198

    14. Peptide-Based Inhibition of miRNA-Guided Gene Silencing

      • Johannes Danner, Balagopal Pai, Ludwig Wankerl, Gunter Meister
      Pages 199-210

    15. Small Molecules Targeting the miRNA-Binding Domain of Argonaute 2: From Computer-Aided Molecular Design to RNA Immunoprecipitation

      • Teresa Bellissimo, Silvia Masciarelli, Elena Poser, Ilaria Genovese, Alberto Del Rio, Gianni Colotti et al.
      Pages 211-221

    16. Surface Plasmon Resonance: A Useful Strategy for the Identification of Small Molecule Argonaute 2 Protein Binders

      • Elena Poser, Ilaria Genovese, Silvia Masciarelli, Teresa Bellissimo, Francesco Fazi, Gianni Colotti
      Pages 223-237

    17. Antagonists of the miRNA-Argonaute 2 Protein Complex: Anti-miR-AGOs

      • Marco F. Schmidt, Oliver Korb, Chris Abell
      Pages 239-249
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Editors and Affiliations

  • BEROCEUTICA GmbH, Potsdam, Germany

    Marco F. Schmidt

Bibliographic Information

  • Book Title: Drug Target miRNA

  • Book Subtitle: Methods and Protocols

  • Editors: Marco F. Schmidt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6563-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-6561-8Published: 07 December 2016

  • Softcover ISBN: 978-1-4939-8236-3Published: 06 July 2018

  • eBook ISBN: 978-1-4939-6563-2Published: 06 December 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 319

  • Number of Illustrations: 32 b/w illustrations, 62 illustrations in colour

  • Topics: Pharmacology/Toxicology

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