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Stem Cell Heterogeneity

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa Hospital Research Institute, Ottawa, Canada

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  • Includes cutting-edge methods and protocols for studying stem cell heterogeneity
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1516)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Heterogeneity of Stem Cells: A Brief Overview

    • Györgyi Műzes, Ferenc Sipos
    Pages 1-12

  3. Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells

    • Sharat Warrier, Mina Popovic, Margot Van der Jeught, Björn Heindryckx
    Pages 13-46

  4. An Effective and Reliable Xeno-free Cryopreservation Protocol for Single Human Pluripotent Stem Cells

    • Guoliang Meng, Anna Poon, Shiying Liu, Derrick E. Rancourt
    Pages 47-56

  5. Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter

    • Leonid V. Bystrykh, Mirjam E. Belderbos
    Pages 57-89

  6. Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells

    • Krzysztof Szade, Karolina Bukowska-Strakova, Monika Zukowska, Alicja Jozkowicz, Józef Dulak
    Pages 91-99

  7. Dissecting Transcriptional Heterogeneity in Pluripotency: Single Cell Analysis of Mouse Embryonic Stem Cells

    • Ana M. V. Guedes, Domingos Henrique, Elsa Abranches
    Pages 101-119

  8. Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs)

    • Josh Cutts, Nicholas Brookhouser, David A. Brafman
    Pages 121-144

  9. Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice

    • Srabani Kar, Shinjini Mitra, Ena Ray Banerjee
    Pages 145-152

  10. Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing

    • Amar M. Singh, Dustin W. Perry, Valeriya V. Adjan Steffey, Kenneth Miller, Daniel W. Allison
    Pages 153-169

  11. Stencil Micropatterning for Spatial Control of Human Pluripotent Stem Cell Fate Heterogeneity

    • Jun Yuan, Geetika Sahni, Yi-Chin Toh
    Pages 171-181

  12. Visualizing the Functional Heterogeneity of Muscle Stem Cells

    • Yasuo Kitajima, Shizuka Ogawa, Yusuke Ono
    Pages 183-193

  13. Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies

    • Chiara Franzin, Martina Piccoli, Luca Urbani, Carlo Biz, Piergiorgio Gamba, Paolo De Coppi et al.
    Pages 195-204

  14. Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells

    • Krzysztof Szade, Monika Zukowska, Alicja Jozkowicz, Jozef Dulak
    Pages 205-217

  15. Growth Factor-Free Pre-vascularization of Cell Sheets for Tissue Engineering

    • Marina Costa, Rogério P. Pirraco, Mariana T. Cerqueira, Rui L. Reis, Alexandra P. Marques
    Pages 219-226

  16. In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry

    • Marc Cloutier, Christine Jobin, Carl Simard, Sonia Néron
    Pages 227-241

  17. Aerosol-Based Cell Therapy for Treatment of Lung Diseases

    • Egi Kardia, Nur Shuhaidatul Sarmiza Abdul Halim, Badrul Hisham Yahaya
    Pages 243-255

  18. Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro

    • Masahide Yoshikawa, Yukiteru Ouji
    Pages 257-267

  19. Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro

    • Yukiteru Ouji, Masahide Yoshikawa
    Pages 269-277

  20. Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro

    • Yukiteru Ouji, Masahide Yoshikawa
    Pages 279-288
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Keywords

About this book

Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity. This goes against the long-held tenet that stem cells, defined by their capacity for self-renewal and lineage development, comprised a homogenous population, thus providing the reader with a new avenue of exploration into the complex world of stem cell study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cell Heterogeneity: Methods and Protocols serves as an ideal guide for investigators exploring this important area of research.  

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Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

About the editor

  

Bibliographic Information

  • Book Title: Stem Cell Heterogeneity

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6550-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2016

  • Hardcover ISBN: 978-1-4939-6549-6Published: 27 August 2016

  • Softcover ISBN: 978-1-4939-8234-9Published: 20 June 2018

  • eBook ISBN: 978-1-4939-6550-2Published: 26 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 390

  • Number of Illustrations: 21 b/w illustrations, 53 illustrations in colour

  • Topics: Cell Biology

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