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Semaphorin Signaling

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Jonathan R. Terman

    1. Departments of Neuroscience and Pharmacology, The University of Texas Southwestern Medical Center, Dallas, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1493)

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About this book

This volume arranged into three sections describes biochemical, in vitro, and in vivo protocols on Semaphorins. Chapters focus on approaches that would allow the novice to study Semaphorins and employ robust assays to characterize mechanisms of action. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Semaphorin Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.



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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Semaphorins and their Signaling Mechanisms

    • Laura Taylor Alto, Jonathan R. Terman
    Pages 1-25

  3. Biochemical Approaches

    1. Front Matter

      Pages 27-27
      Download chapter PDF

    2. Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells

      • Joanne Soong, Glynis A. Scott
      Pages 29-40

    3. Expression and Purification of Class 7 Semaphorin and Its PlexinC1 Receptor Using Baculovirus-Mediated Mammalian Cell Gene Transduction

      • Xiaoyan Chen, Po-Han Chen, Xiaolin He
      Pages 41-56

    4. Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System

      • Terukazu Nogi, Emiko Mihara, Norihisa Yasui, Junichi Takagi
      Pages 57-72

    5. Plate-Based Assay for Measuring Direct Semaphorin–Neuropilin Interactions

      • Matthew W. Parker, Craig W. Vander Kooi
      Pages 73-87

    6. Characterizing Plexin GTPase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry, and Isothermal Titration Calorimetry

      • Jeannine Muller-Greven, SoonJeung Kim, Prasanta K. Hota, Yufeng Tong, Susmita Borthakur, Matthias Buck
      Pages 89-105

    7. In Vitro Assay for the Rap GTPase-Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin

      • Heath G. Pascoe, Yuxiao Wang, Xuewu Zhang
      Pages 107-118

    8. Characterizing F-actin Disassembly Induced by the Semaphorin-Signaling Component MICAL

      • Jimok Yoon, Ruei-Jiun Hung, Jonathan R. Terman
      Pages 119-128

    9. Characterizing ErbB-2-Mediated Tyrosine Phosphorylation and Activation of Plexins

      • Thomas Worzfeld, Jakub M. Swiercz
      Pages 129-146

    10. Characterizing PKA-Mediated Phosphorylation of Plexin Using Purified Proteins

      • Taehong Yang, Jonathan R. Terman
      Pages 147-159

  4. In Vitro Approaches

    1. Front Matter

      Pages 161-161
      Download chapter PDF

    2. Using Heterologous COS-7 Cells to Identify Semaphorin-Signaling Components

      • Atsuko Sakurai, Colleen L. Doçi, J. Silvio Gutkind
      Pages 163-170

    3. Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition

      • Lionel A. T. Meyer, Andrius Kaselis, Saulius Satkauskas, Dominique Bagnard
      Pages 171-183

    4. Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse

      • Paul C. Bridgman
      Pages 185-194

    5. An Electrical Impedance-Based Method for Quantitative Real-Time Analysis of Semaphorin-Elicited Endothelial Cell Collapse

      • Chiara Camillo, Noemi Gioelli, Federico Bussolino, Guido Serini
      Pages 195-207

    6. Regulation of Cortical Dendrite Morphology and Spine Organization by Secreted Semaphorins: A Primary Culture Approach

      • Sheng-Shiang Peng, Tracy S. Tran
      Pages 209-222

    7. Characterizing Semaphorin Signaling Using Isolated Neurons in Culture

      • Sophie Chauvet, Erik Mire, Fanny Mann
      Pages 223-235

    8. Performing Axon Orientation Assays with Secreted Semaphorins and Other Guidance Cues

      • Céline Delloye-Bourgeois, Frédéric Moret, Valérie Castellani
      Pages 237-246
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Editors and Affiliations

  • Departments of Neuroscience and Pharmacology, The University of Texas Southwestern Medical Center, Dallas, USA

    Jonathan R. Terman

Bibliographic Information

  • Book Title: Semaphorin Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Jonathan R. Terman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6448-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6446-8Published: 28 October 2016

  • Softcover ISBN: 978-1-4939-8203-5Published: 14 July 2018

  • eBook ISBN: 978-1-4939-6448-2Published: 27 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 503

  • Number of Illustrations: 42 b/w illustrations, 54 illustrations in colour

  • Topics: Protein Science

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