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Germ-Free Mice Model for Studying Host–Microbial Interactions

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Mouse Models for Drug Discovery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1438))

Abstract

Germ-free (GF) mice are a relevant model system to study host–microbial interactions in health and disease. In this chapter, we underscore the importance of using GF mice model to study host–microbial interactions in obesity, immune development and gastrointestinal physiology by reviewing current literature. Furthermore, we also provide a brief protocol on how to setup a gnotobiotic facility in order to properly maintain and assess GF status in mice colonies.

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Authors and Affiliations

  1. Department of Gastroenterology and Hepatology, Mayo Clinic, 200 First St. SW, Rochester, MN, 55905, USA

    Yogesh Bhattarai & Purna C. Kashyap M.B.B.S.

Authors
  1. Yogesh Bhattarai
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  2. Purna C. Kashyap M.B.B.S.
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Correspondence to Purna C. Kashyap M.B.B.S. .

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Editors and Affiliations

  1. Takeda Pharmaceutical Company, Cambridge, Massachusetts, USA

    Gabriele Proetzel

  2. The Jackson Laboratory, Bar Harbor, Maine, USA

    Michael V. Wiles

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Bhattarai, Y., Kashyap, P.C. (2016). Germ-Free Mice Model for Studying Host–Microbial Interactions. In: Proetzel, G., Wiles, M. (eds) Mouse Models for Drug Discovery. Methods in Molecular Biology, vol 1438. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3661-8_8

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  • DOI: https://doi.org/10.1007/978-1-4939-3661-8_8

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  • Print ISBN: 978-1-4939-3659-5

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