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Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols

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Molecular Genetics of Asthma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1434))

Abstract

The RNA interference (RNAi) plays an important role in regulation of gene expression. It is a mechanism used by many organisms to silence the expression of genes that control different processes in the cell. The double strand (ds) RNA molecule inhibits gene expression of a targeted gene with high specificity and selectivity.

Different types of small ribonucleic acid molecules, microRNA (miRNA), small interfering RNA (siRNA), short hairpin RNA (shRNA), and the piwi RNA (piRNA) are involved in the RNA interference. RNAi is a relevant research tool in cell cultures and in vivo experiments because synthetic dsRNA introduced into cells can selectively silence specific target genes.

Here, we describe a general guide for gene silencing mediated by siRNA, focusing on the most used delivery methods: lipid-mediated and electroporation transfection.

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Author information

Authors and Affiliations

  1. Department of Biomedical and Diagnostic Sciences, University of Salamanca, Salamanca, Spain

    Asunción García-Sánchez

  2. Salamanca Institute for Biomedical Research (IBSAL), UniversityHospital of Salamanca, Salamanca, Spain

    Asunción García-Sánchez

  3. Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain

    Fernando Marqués-García

  4. Department of Clinical Biochemistry, University Hospital of Salamanca, Salamanca, Spain

    Fernando Marqués-García

Authors
  1. Asunción García-Sánchez
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  2. Fernando Marqués-García
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Corresponding author

Correspondence to Asunción García-Sánchez .

Editor information

Editors and Affiliations

  1. Department of Clinical Biochemistry, University Hospital of Salamanca, Salamanca, Spain

    María Isidoro García

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García-Sánchez, A., Marqués-García, F. (2016). Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols. In: Isidoro García, M. (eds) Molecular Genetics of Asthma. Methods in Molecular Biology, vol 1434. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3652-6_10

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  • DOI: https://doi.org/10.1007/978-1-4939-3652-6_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3650-2

  • Online ISBN: 978-1-4939-3652-6

  • eBook Packages: Springer Protocols

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