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Type-1 Diabetes

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Kathleen M. Gillespie

    1. Diabetes and Metabolism Unit, School of Clinical Sciences, Southmead Hospital, University of Bristol, Bristol, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1433)

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About this book

The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers.   Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.


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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Type 1 Diabetes: Current Perspectives

    • Aizhan Kozhakhmetova, Kathleen M. Gillespie
    Pages 1-9

  3. Type 1 Diabetes High-Risk HLA Class II Determination by Polymerase Chain Reaction Sequence-Specific Primers

    • Rachel J. Aitken, Georgina L. Mortimer, Kathleen M. Gillespie
    Pages 13-20

  4. Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants

    • Izortze Santin, Reinaldo S. Dos Santos, Decio L. Eizirik
    Pages 21-54

  5. Islet Autoantibody Analysis: Radioimmunoassays

    • Rebecca Wyatt, Alistair J. K. Williams
    Pages 57-83

  6. Islet Autoantibody Detection by Electrochemiluminescence (ECL) Assay

    • Liping Yu
    Pages 85-91

  7. Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function

    • T. J. McDonald, M. H. Perry
    Pages 93-102

  8. Histology of Type 1 Diabetes Pancreas

    • Abby Willcox, Kathleen M. Gillespie
    Pages 105-117

  9. Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes

    • James A. Pearson, F. Susan Wong
    Pages 119-125

  10. Tracking Immunological Responses of Islet Antigen-Specific T Cells in the Nonobese Diabetic (NOD) Mouse Model of Type 1 Diabetes

    • Terri C. Thayer, F. Susan Wong
    Pages 127-134

  11. Adoptive Transfer of Autoimmune Diabetes Using Immunodeficient Nonobese Diabetic (NOD) Mice

    • Evy De Leenheer, F. Susan Wong
    Pages 135-140

  12. Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes

    • Mary N. Dang, Claire M. Bradford, Paolo Pozzilli, R. David Leslie
    Pages 143-151

  13. Fluorescence In Situ Hybridization with Concomitant Immunofluorescence in Human Pancreas

    • Jody Ye, Kathleen M. Gillespie
    Pages 153-158

  14. Laser Capture and Single Cell Genotyping from Frozen Tissue Sections

    • Thomas Kroneis, Jody Ye, Kathleen Gillespie
    Pages 159-167

  15. The Gut Microbiome in the NOD Mouse

    • Jian Peng, Youjia Hu, F. Susan Wong, Li Wen
    Pages 169-177

  16. Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes

    • Karen T. Elvers, Alistair J. K. Williams
    Pages 179-207

  17. Back Matter

    Pages 209-213
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Editors and Affiliations

  • Diabetes and Metabolism Unit, School of Clinical Sciences, Southmead Hospital, University of Bristol, Bristol, United Kingdom

    Kathleen M. Gillespie

Bibliographic Information

  • Book Title: Type-1 Diabetes

  • Book Subtitle: Methods and Protocols

  • Editors: Kathleen M. Gillespie

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3643-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2016

  • Hardcover ISBN: 978-1-4939-3641-0Published: 03 June 2016

  • Softcover ISBN: 978-1-4939-8104-5Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3643-4Published: 03 June 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 213

  • Number of Illustrations: 11 b/w illustrations, 33 illustrations in colour

  • Topics: Molecular Medicine

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