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In Vivo Analysis of Intestinal Mononuclear Phagocytes

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Book cover Dendritic Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1423))

Abstract

The study of the intestinal dendritic cell (DC) compartment, its homeostasis, regulation, and response to challenges calls for the investigation within the physiological tissue context comprising the unique anatomic constellation of the epithelial single cell layer and the luminal microbiota, as well as neighboring immune and nonimmune cells. Here we provide protocols we developed that use a combination of conditional cell ablation, conditional compartment mutagenesis, and adoptive precursor transfers to study DC and other intestinal mononuclear phagocytes in in vivo context. We will highlight pitfalls and strengths of these approaches.

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Acknowledgments

We would like to thank the past and present members of the Jung lab for sharing their protocols. This work was supported by the European Research Council (340345).

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Authors and Affiliations

  1. Department of Immunology, The Weizmann Institute of Science, Rehovot, 76100, Israel

    Caterina Curato, Biana Bernshtein, Tegest Aychek & Steffen Jung

Authors
  1. Caterina Curato
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  2. Biana Bernshtein
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  3. Tegest Aychek
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  4. Steffen Jung
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Corresponding author

Correspondence to Steffen Jung .

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Editors and Affiliations

  1. Pavillon Pasteur, Institut Curie INSERM U932, Paris Cedex 05, France

    Elodie Segura

  2. Department of Biodefense, Tokyo Medical and Dental University, Tokyo, Japan

    Nobuyuki Onai

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Curato, C., Bernshtein, B., Aychek, T., Jung, S. (2016). In Vivo Analysis of Intestinal Mononuclear Phagocytes. In: Segura, E., Onai, N. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 1423. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3606-9_18

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  • DOI: https://doi.org/10.1007/978-1-4939-3606-9_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3604-5

  • Online ISBN: 978-1-4939-3606-9

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