Skip to main content
Springer Nature Link
Log in

Optogenetics

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Arash Kianianmomeni

    1. University of Bielefeld, Dept of Cellular & Dev Biology of Plants, Bielefeld, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1408)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

About this book

This volume provides researchers with up-to-date protocols and takes a close look at current research and promising applications. Optogenetics: Methods and Protocols contains a collection of recently developed technical protocols on optogenetic applications in neuroscience, brain mapping, treatment of neurological disorders, and restoration of visual function. Several introductory and discussion chapters offer a wide overview about sources and diversity of optogenetic tools, design strategies, and potential applications in other fields like plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers an interdisciplinary view of optogenetic applications and its potential to develop as a valuable tool for basic research, as well as biomedical and biotechnological applications.

 

Similar content being viewed by others

Keywords

Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Optogenetics: Basic Concepts and Their Development

    • Yong Ku Cho, Dan Li
    Pages 1-17

  3. Natural Resources for Optogenetic Tools

    • Tilo Mathes
    Pages 19-36

  4. Algal Photobiology: A Rich Source of Unusual Light Sensitive Proteins for Synthetic Biology and Optogenetics

    • Arash Kianianmomeni, Armin Hallmann
    Pages 37-54

  5. Reversible Photoregulation of Gene Expression and Translation

    • Shinzi Ogasawara
    Pages 55-66

  6. Controlling Protein Activity and Degradation Using Blue Light

    • Anne P. Lutz, Christian Renicke, Christof Taxis
    Pages 67-78

  7. Photo Control of Protein Function Using Photoactive Yellow Protein

    • Jakeb M. Reis, G. Andrew Woolley
    Pages 79-92

  8. A Fluorometric Activity Assay for Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators

    • Charlotte Helene Schumacher, Heinz G. Körschen, Christopher Nicol, Carlos Gasser, Reinhard Seifert, Martin Schwärzel et al.
    Pages 93-105

  9. Optogenetic Control of Pancreatic Islets

    • Thomas M. Reinbothe, Inês G. Mollet
    Pages 107-123

  10. Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression

    • Rocio Ochoa-Fernandez, Sophia L. Samodelov, Simon M. Brandl, Elke Wehinger, Konrad Müller, Wilfried Weber et al.
    Pages 125-139

  11. Enhancing Channelrhodopsins: An Overview

    • Jonas Wietek, Matthias Prigge
    Pages 141-165

  12. Optogenetics in Drosophila Neuroscience

    • Thomas Riemensperger, Robert J. Kittel, André Fiala
    Pages 167-175

  13. Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches

    • Damien Lemoine, Romain Durand-de Cuttoli, Alexandre Mourot
    Pages 177-193

  14. Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats

    • Kedi Xu, Jiacheng Zhang, Songchao Guo, Xiaoxiang Zheng
    Pages 195-206

  15. Combined Optogenetic and Chemogenetic Control of Neurons

    • Ken Berglund, Jack K. Tung, Bryan Higashikubo, Robert E. Gross, Christopher I. Moore, Ute Hochgeschwender
    Pages 207-225

  16. Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex

    • Christopher Pawela, Edgar DeYoe, Ramin Pashaie
    Pages 227-241

  17. An Optimized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in Primary Neurons

    • Shiyao Wang, Yong Ku Cho
    Pages 243-249

  18. Optogenetic Approaches for Mesoscopic Brain Mapping

    • Michael Kyweriga, Majid H. Mohajerani
    Pages 251-265

  19. Optogenetic Tools for Confined Stimulation in Deep Brain Structures

    • Alexandre Castonguay, Sébastien Thomas, Frédéric Lesage, Christian Casanova
    Pages 267-279

  20. Remote Patterning of Transgene Expression Using Near Infrared-Responsive Plasmonic Hydrogels

    • Francisco Martín-Saavedra, Nuria Vilaboa
    Pages 281-292
Back to top

Editors and Affiliations

  • University of Bielefeld, Dept of Cellular & Dev Biology of Plants, Bielefeld, Germany

    Arash Kianianmomeni

Bibliographic Information

  • Book Title: Optogenetics

  • Book Subtitle: Methods and Protocols

  • Editors: Arash Kianianmomeni

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3512-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3510-9Published: 11 March 2016

  • Softcover ISBN: 978-1-4939-8062-8Published: 07 June 2018

  • eBook ISBN: 978-1-4939-3512-3Published: 10 March 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 408

  • Number of Illustrations: 13 b/w illustrations, 98 illustrations in colour

  • Topics: Neurosciences, Human Genetics

Publish with us

Policies and ethics

Back to top